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Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.


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Journal ArticleDOI
TL;DR: In this article, the authors investigated whether the foliar application of kinetin (KN), a kind of cytokinins alleviates cadmium toxicity in Solanum melongena L. seedlings.

109 citations

Journal ArticleDOI
TL;DR: The presence of taxol in callus samples was established by high performance liquid chromatography, its biological activity confirmed by a microtubule-stabilizing bioassay and its structure confirmed using one-and two-dimensional 1H and 13C nuclear magnetic resonance spectroscopy.
Abstract: Callus was induced from Taxus baccata cv. Repandens Parsons ex Rehd., T. brevifolia Nutt., T. cuspidata Sieb. & Zucc., and T. x media cvs. Hicksii and Densiformis Rehd. using different concentrations of 2,4-d-(2,4-dichlorophenoxyacetic acid), IBA (indole-3-butyric acid), or NAA α-naphthalene acetic acid in combination with kinetin. All cultures grew slowly following the first subculture, and a majority turned brown and ceased growth within the next six to twelve months. The callus cultures which lived, continued to grow very slowly for one to two years before the growth rate improved. Initiation of roots and shoot primordia-like structures occurred on some cultures maintained in the dark, and 16 h light/8 h dark, respectively. A fast-growing, habituated callus line (CR-1) derived from T. x media Rehd. cv. Hicksii was established from callus initiated in 1986. Supplementing the medium with casein hydrolysate and both fructose and glucose enhanced the growth rate. A great deal of heterogeneity was found among and within the callus, with respect to the amount of taxol produced. The callus exhibited levels of taxol ranging from 0.1 to 13.1 mg kg-1 (0.0001 to 0.0131%) on a dry weight basis. Overall, the older brown-colored callus produced more taxol than the younger pale yellow-colored callus. The presence of taxol in callus samples was established by high performance liquid chromatography, its biological activity confirmed by a microtubule-stabilizing bioassay and its structure confirmed using one-and two-dimensional 1H and 13C nuclear magnetic resonance spectroscopy.

108 citations

Journal ArticleDOI
TL;DR: A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the identification and quantification of Kinetin and kinetin riboside in purified coconut water extract sample.

107 citations

Journal ArticleDOI
01 Sep 1967-Planta
TL;DR: The carrot-root tissue culture assay for cytokinin activity has been improved by changing the site of explant excision and eliminating certain vitamins from the basal medium, which increased its sensitivity and enabled zeatin to be detected at concentrations less than 5×10-5μM.
Abstract: The carrot-root tissue culture assay for cytokinin activity has been improved by changing the site of explant excision and eliminating certain vitamins from the basal medium. These modifications increased its sensitivity and enabled zeatin [6-(4-hydroxy-3-methylbut-trans-2-enyl)aminopurine] to be detected at concentrations less than 5×10(-5)μM. In the improved assay, zeatin was markedly more active than kinetin, 6-benzylaminopurine, 6-(o-methylbenzyl)aminopurine and 6-(3-methylbut-2-enyl)aminopurine.The activity of zeatin also exceeded that of kinetin in the etiolated bean-leaf disk expansion assay. Zeatin was markedly more effective than kinetin and 6-(3-methylbut-2-enyl)aminopurine in promoting frond expansion and increasing frond number of Spirodela oligorrhiza cultures grown under continuous illumination. Zeatin was also more active than kinetin and 6-(3-methylbut-2-enyl)aminopurine in increasing frond number of Spirodela cultures grown in darkness. In retarding the senescence of disks of leaves of several species, kinetin was considerably more effective than zeatin which was more active than 6-(3-methylbut-2-enyl)aminopurine. The allylic hydroxyl group in zeatin is therefore a structural feature associated with high cytokinin activity.The relative activities of cytokinins can be very different and even in reverse order in different bioassays. It is suggested that this is due to the mechanism of cytokinin action varying in the different biological systems used.

105 citations

Journal ArticleDOI
TL;DR: Neither NAA nor kinetin at any concentration tested stimulated tracheary element formation in the absence of an effective level of the other hormone, however, 2,4-D at 10(-7) or 10(-6)m promoted both cell proliferation and tracheARY element differentiation in the presence of an exogenous cytokinin.
Abstract: The relationship between tracheary element differentiation, cell proliferation and growth hormones was examined in agar-grown soybean callus. The time course of cell division and tracheary element formation in tissues grown on a medium containing 5 × 10−7m kinetin and 10−5m NAA was determined by means of maceration technique. After a slight lag period, a logarithmic increase in cell number was observed through the twelfth day of the culture period. Cell numbers increased at a considerably slower rate after the twelfth day. The rate of tracheary element formation varied with the rate of cell proliferation. Tracheary elements increased logarithmically during the log phase of growth. As the rate of cell division decreased after the twelfth day of culture, the rate of tracheary element formation also decreased. In the presence of 10−5m NAA, cell number increased as the kinetin concentration was increased between 10−9 and 10−6m. However, tracheary element formation was not initiated unless the kinetin concentration was 5 × 10−8m or above. When the Biloxi callus was subcultured repeatedly on media containing 10−8m kinetin, a tracheary element-free population of cells was obtained. This undifferentiated tissue produced tracheary elements upon transfer to a medium containing 5 × 10−7m kinetin. In the presence of 5 × 10−7m kinetin, NAA stimulated cell proliferation between 10−7 and 10−5m, but no tracheary elements were formed without auxin, or with 10−7m NAA. Neither NAA nor kinetin at any concentration tested stimulated tracheary element formation in the absence of an effective level of the other hormone. However, 2,4-D at 10−7 or 10−6m promoted both cell proliferation and tracheary element differentiation in the absence of an exogenous cytokinin.

105 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023115
2022243
2021139
2020137
2019156
2018189