Topic
Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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TL;DR: The expression of LHCP-gene activity, in response to kinetin addition to tobacco cell suspension cultures, is regulated by the level of pLHCP-encoding mRNA rather than by translational or post-translational controls.
Abstract: Tobacco cell suspension cultures responded to cytokinins (for instance kinetin) by full chloroplast differentiation. The hormone had the effect of stimulating the appearance of a few prominent plastid proteins. Synthesis of the light-harvesting chlorophyl a/b-binding protein (LHCP) in response to kinetin was noteworthy (Axelos M. et al.: Plant Sci Lett 33:201–212, 1984). Poly(A)+RNAs were prepared from cells grown in the presence of or without added kinetin. Poly(A)+RNA recovery and translation activity were not quantitatively altered by the hormone treatment. In vitro translation of polyadenylated mRNA into precursor polypeptides of LHCP (pLHCP) was quantified by immunoprecipitation and SDS-PAGE fractionation of pLHCP immunoprecipitates: pLHCP-mRNA translating activity was found to be stimulated in parallel to mature LHCP accumulation by kinetin-induced cells. Dot-blot and northern-blot hybridizations of poly(A)+RNA were carried out, using as a probe a pea LHCP-cDNA clone (Broglie R. et al.: Proc Natl Acad Sci USA 78: 7304–7308, 1981). A ten-fold increase of the level of pLHCP-encoding sequences was observed in poly(A)+RNA prepared from 9-d kinetin-stimulated cells, compared to control cells. Oligo(dT)-cellulose-excluded RNA fractions exhibited very low hybridization levels, in the same ratios as those obtained with poly(A)+RNA. Thus, the expression of LHCP-gene activity, in response to kinetin addition to tobacco cell suspension cultures, is regulated by the level of pLHCP-encoding mRNA rather than by translational or post-translational controls. re]19850218 rv]19850605 ac]19850613
95 citations
TL;DR: The effect of cytokinins (kinetin and trans-zeatin) on LR formation in rice (Oryza sativa L.) showed that cytokinin has an inhibitory effect on LR initiation and stimulatory effect onLR elongation, suggesting that cytkinin acts on LR elongation through an auxin dependent pathway.
94 citations
TL;DR: A natural free radical scavenger, ascorbic acid (AsA) shows both counteractive and enhancing effects on JA‐inducible phytoalexin production, depending on its concentration, which suggests that active oxygen species (AOS) may play important roles in phy toalex in production by JA in rice leaves.
94 citations
TL;DR: Growth and podophyllotoxin production of callus tissues were examined under various culture conditions as discussed by the authors, showing that IAA and/or NAA in the presence of kinetin and casamino acids promoted callus growth.
94 citations
TL;DR: In this paper, a trial conducted in 1974, foliar application of an aqueous seaweed extract of known cytokinin activity produced a significant increase in the yield of potatoes of the variety King Edward.
Abstract: In a trial conducted in 1974, foliar application of an aqueous seaweed extract of known cytokinin activity produced a significant increase in the yield of potatoes of the variety King Edward. A synthetic cytokinin, kinetin, also produced significant increases in the yield of tubers of both the varieties King Edward and Pentland Dell. There was close correlation between the results from the use of kinetin and the seaweed extract of equivalent cytokinin activity, which suggests that the effect of the seaweed extract was due to its cytokinin content. In a complementary trial in 1975 with King Edward potatoes, significant increases were obtained again in tuber yields through treatment with aqueous seaweed extract. The major increase was in ware potatoes.
93 citations