Topic
Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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TL;DR: A new multiple hanging-drop assay has been tested with a growth inhibitor, cycloheximide, and a growth-promoter, fusicoccin to examine the growth-response of pollen-tubes of Prunus avium to brassinolide, a naturally occurring promoter of plant growth.
Abstract: A new multiple hanging-drop assay has been tested with a growth inhibitor, cycloheximide, and a growth-promoter, fusicoccin. This assay has been used to examine the growth-response of pollen-tubes of Prunus avium to brassinolide, a naturally occurring promoter of plant growth. This response is compared with that of indol-3-ylacetic acid, gibberellic acid, and kinetin. Pollen tubes responded to brassinolide and fusicoccin at 1 nM and above, a concentration one order of magnitude lower than that for indol-3-ylacetic acid and gibberellic acid. Kinetin did not stimulate growth of the pollen tubes.
76 citations
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TL;DR: The induction with senescence, and the repression with kinetin and glucosamine, suggest chat chloroplast isoperoxidase C4 may function as a chlorophyll-degrading enzyme during barley leaf senescences.
Abstract: Total peroxidase activity increased during senescence of excised barley (Hordeum vulgare L. cv. Kashimamugi) leaves. Kinetin treatment furter increased total peroxidase activity but repressed chlorophyll degradation in excised barley leaves. When isoperoxidases were extracted from barley leaf segments. 4 cationic and 4 anionic isozymes were found in polyacrylamide gel electrophorests during leaf senescence. The chloroplasts contained only two cationic isoperoxidase activities. One (designated C4) was repressed by kinetin. and the other (C3) was increased by kinetin. Glucosamine, which also repressed the degradation of chlorophyll, completely repressed C4 activity but did not affect C3 activity. The induction with senescence, and the repression with kinetin and glucosamine, suggest chat chloroplast isoperoxidase C4 may function as a chlorophyll-degrading enzyme during barley leaf senescence.
75 citations
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TL;DR: Two methods of in vitro culture were employed to regenerate papaya plants, one involved regeneration of plants from callus and the other, production of multiple plants from single shoot-tip explants.
Abstract: Two methods of in vitro culture were employed to regenerate papaya plants. One involved regeneration of plants from callus and the other, production of multiple plants from single shoot-tip explants. Callus was induced from stem sections of papaya seedlings in a medium containing 1 mg per 1 NAA and 0.1 mg per 1 kinetin. The callus regenerated shoots and/or embryoids when transferred to a medium of lower auxin, 0 to 0.05 mg per 1 IAA, and higher cytokinin, 1 to 2 mg per 1 kinetin. Multiple shoots were produced when the excised shoot-tip explants were cultured in a medium supplemented with 0.05 mg per 1 IAA and either 5 mg per 1 kinetin or 0.5 to 1.0 mg per 1 benzyladenine. Root formation of the shoots or embryoids that derived from callus or shoot tips occurred in a medium containing 5 mg per 1 IAA and in a light intensity of 3000 to 4000 lx. The rooted plants could be established in soil and under standard greenhouse conditions after they had been acclimated by initially growing them in moist vermiculite contained in polyethylene-covered pots.
75 citations
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TL;DR: The addition of silver nitrate to the induction medium also significantly improved the percentage of regeneration in three genotypes tested, and maximum bud development and elongation were achieved when kinetin in the range 1–2 mg l −1 was used.
75 citations
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TL;DR: A simple procedure for regeneration of cucumber plants from cotyledon and hypocotyl explants has been developed and eighty-five percent of the mature somatic embryos formed showed a typical bipolar structure.
Abstract: A simple procedure for regeneration of cucumber plants (Cucumis sativus L. cv. Poinsett 76) from cotyledon and hypocotyl explants has been developed. Somatic embryogenesis was induced on Murashige and Skoog (MS) salts and vitamins medium supplemented with 2,4-D at 2.0 mg·liter -1 and kinetin at 0.5 mg·liter -1 . Development of embryos was accomplished on MS medium with NAA at 1.0 mg·liter -1 and kinetin at 0.5 mg·liter -1 . Eighty-five percent of the mature somatic embryos formed showed a typical bipolar structure. All developed into morphologically normal plantlets when transferred to MS medium containing no growth regulators. Chemical name used: 2,4- dichlorophenoxyacetic acid (2,4-D). Previous reports have described proce- dures for cucumber plant regeneration from leaf callus (Malepszy and Nadolska-Orczyk, 1983; Nadolska-Orczyk and Malepszy, 1984),
75 citations