Topic
Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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TL;DR: The concentrations of kinetin and IAA used in these experiments were similar to those used to induce cell proliferation in differentiated areas of the pea root; therefore, it is probable that controlling sites or moieties are activated or derepressed in cells of differentiated tissue and inactivated or repressed in meristematic cells by the direct or indirect action of the chemicals.
72 citations
TL;DR: Abscisic acid inhibits stomatal opening in isolated abaxial epidermal strips of Vicia faba and appears to act directly on the guard cells, rather than by influencing water potentials throughout the leaf.
Abstract: Abscisic acid inhibits stomatal opening in isolated abaxial epidermal strips of Vicia faba. Kinetin, benzyladenine, and gibberellic acid, which can enhance stomatal apertures in intact leaves, are ...
72 citations
TL;DR: A successful procedure was established for in vitro plant regeneration from callus derived from stem and leaf explants of Centella asiatica on semisolid modified Murashige and Skoog's medium supplemented with 2.0mg L3 kinetin and 4.0 mg L3 a-naphthaleneacetic acid.
Abstract: A successful procedure was established for in vitro plant regeneration from callus derived from stem and leaf explants of Centella asiatica on semisolid modified Murashige and Skoog's [7] medium supplemented with 2.0 mg L3 kinetin and 4.0 mg L3 a-naphthaleneacetic acid. The rate of shoot-bud regeneration was the highest (42.8 and 54.3 shoots/culture in stem and leaf derived callus respectively) after 4 weeks of subculture on 4.0 mg L3 6-benzyladenine, 2.0 mg L3 Kn, 0.25 mg L3 a-naphthaleneacetic acid and 20 mg L3 adenine sulfate. Differentiated shoots rooted within 11 days in 1/2 strength MS basal salts supplemented with 0.5 mg L3 indole-3-acetic acid and 2% (w/v) sucrose. About 85% of rooted plantlets were acclimatized and transferred to the greenhouse.
72 citations
TL;DR: Out of 6-benzyl aminopurine (BAP), kinetin (KIN), N (Δ−2 isopentyl) adenine (2iP) andAdenine sulphate (AS), only BAP and KIN were found to be more effective in enhancing the frequency of shoot regeneration.
Abstract: Conditions for plant regeneration from excised cotyledons of Vigna radiata were studied. Complete plant developed from the uncallused proximal ends of cotyledons on Murashige & Skoog's (MS), Gamborg's (B5) and ‘C’ (MS salts + B5 vitamins) basal media. The basal medium ‘C’ was found to be best for plant regeneration. Regeneration frequency, however, varied with genotype, size, orientation and age of explant and the different plant growth regulators combination in the medium. Addition of cytokinins induced callusing at the proximal ends of cotyledons followed by multiple shoot formation. Out of 6-benzyl aminopurine (BAP), kinetin (KIN), N (Δ−2 isopentyl) adenine (2iP) and adenine sulphate (AS), only BAP and KIN were found to be more effective in enhancing the frequency of shoot regeneration. BAP at 1×10-1M induced maximum (60%) shoot regeneration whereas maximum number of shoots (8 to 9 shoots) per explant was observed with 5×10-6M BAP. Cotyledons excised from two-day old seedlings were most regenerative. The regenerative response of cotyledons decreased when sliced into two equal parts either longitudinally or transversely. Callusing and organogenic differentiation occured only if the petiolar end of cotyledons was in contact with medium. None of the tested treatments were effective in inducing shoot bud differentiation from subcultured callus. Well developed shoots rooted when incubated on half strength MS, MS and MS basal medium supplemented with IAA (5×10-6M). The rooted plants were transferred to pots and later established in the field with 60% success.
72 citations
TL;DR: While galactose and lactose over a broad concentration range enhanced embryogenesis in all calli studied, sucrose supported embryogenesis only at the lower levels tested (8–32 mM with various calli).
72 citations