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Kinetin
About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.
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TL;DR: Results suggest that kinetin has effective free radical-scavenging activity in vitro and antithrombotic activity in vivo and may be a potential therapeutic agent for arterial thrombosis, but its toxicity must be further assessed.
69 citations
TL;DR: It was shown that adenine and various related compounds can stimulate bud formation and kinetin is especially effective in promoting cell division in buds but not in the protonemata; a growth-inhibitory material is built up in the medium during the growth of the moss.
Abstract: 1. The effect of various factors leading up to development of the gametophore of Tortella caespitosa was investigated with special interest centered on auxins and nucleic acid derivatives. 2. Earlier work showing the inhibition of budding by high concentrations of auxins and the stimulatory effect of low concentrations was confirmed. 3. It was shown that (a) adenine and various related compounds can stimulate bud formation; (b) kinetin is especially effective in promoting cell division in buds but not in the protonemata; (c) a growth-inhibitory material is built up in the medium during the growth of the moss. 4. A mechanism is suggested for the formation of the gametophore.
69 citations
Journal Article•
TL;DR: This method could be useful for regenerating large number of plants as well as provide a target tissue for genetic transformation studies after induction of direct embryogenesis from inflorescence segments in Indian sugarcane.
Abstract: A protocol for direct somatic embryogenesis without an intervening callus phase was developed for sugarcane (Saccharum spp. hybrids) using immature inflorescence segments. Murashige and Skoog (MS) medium supplemented with0.5 mg/l naphthaleneacetic acid, 2.5 mg/I kinetin, 100 mg/l L-glutamine and 4% sucrose showed high frequency of somatic embryo development (54.09 ′ 2.7%), with an average 7.72 ′ 0.89 plants per explant. Embryo development was seen all over the cultured explants within four weeks of culture and the embryos germinated within a week upon transfer to basal MS medium without any growth regulators and all the plants grew normally in the greenhouse. This is a report on the induction of direct embryogenesis from inflorescence segments in Indian sugarcane. This method could be useful for regenerating large number of plants as well as provide a target tissue for genetic transformation studies.
69 citations
TL;DR: Some somatic embryos converted into normal plants capable of greenhouse survival when induced on peanut leaflets from aseptically germinated embryo axes, and other treatments had lower percent embryos, no secondary embryogenesis, and embryos with single axes with two cotyledons.
Abstract: Somatic embryos were induced on peanut (Arachis hypogaea) leaflets from aseptically germinated embryo axes Leaflet size influenced percent somatic embryogenesis; 5–8 mm long cut leaflets were superior to 2–3 mm long uncut leaflets Maximum embryogenesis of 146% was obtained after a 15 d incubation on induction medium (modified MS with B5 vitamins, 30 g/l sucrose, 4 g/l Gel-Gro, 40 mg/l 2,4-D +02 mg/l kinetin) followed by transfer to a secondary medium with 5 mg/l 2,4-D+02 mg/l kinetin Primary somatic embryos were fused along the axes with no distinct cotyledons, but secondary embryos had single axes with two cotyledons Other treatments had lower percent embryogenesis, no secondary embryogenesis, and embryos with single axes with two cotyledons Some somatic embryos converted into normal plants capable of greenhouse survival
69 citations
TL;DR: Although regeneration of soybean plants was not achieved, these observations suggest that it may be achievable, and Concentrations and chemical forms of the growth regulators in liquid and solidified media were altered in an attempt to achieve in vitro plant regeneration.
Abstract: Glycine max (L.) Merr. (soybean) andGlycine soja Sieb. and Zucc. cell suspension cultures were grown and used as inoculum sources for growing callus on agar-solidified nutrient media. Concentrations and chemical forms of the growth regulators in liquid and solidified media were altered in an attempt to achieve in vitro plant regeneration. Numerous embryoids, particularly ofG. soja, were produced on basal nutrient media supplemented with 100 ppm casein hydrolysate, 0.1 μM abscisic acid, 2.25 μM 2,4-dichlorophenoxyacetic acid, and 15 μM adenine or 0.46 μM kinetin. Often the roots of the embryoids elongated. This was enhanced in the presence of an inhibitor of gibberellin synthesis (1 to 20 μM Amo 1618). Callus recovered from aG. soja suspension culture produced one shoot structure when grown on a solid medium containing 0.2 μM Amo 1618 and 80 μM glutathione. The shoot structure consisted of two distinct buds, one producing two leaves. The shoot did not develop into a plant. Although regeneration of soybean plants was not achieved, these observations suggest that it may be achievable.
69 citations