Kinetin

About: Kinetin is a research topic. Over the lifetime, 7856 publications have been published within this topic receiving 135550 citations. The topic is also known as: Kinetin.

In vitro gynogenesis in Beta vulgaris L.: effects of plant growth regulators, temperature, genotypes and season

Abstract: In order to increase the yield of haploid sugarbeet plants obtained from in vitro culture of unfertilized ovules, we have studied several factors: (1) the nature and concentration of various growth hormones, (2) the temperature for the cultured ovules, (3) genotypes and (4) seasonal effects. We found N6 medium [7] supplemented with 2.85 μM of indoleacetic acid (IAA) and 0.88 μM of 6-benzylaminopurine (BAP) or N6 medium containing 2.3 μM of kinetin (KIN) to give the best qualitative and quantitative results for the culture of ovules. A two-step process for the induction and the development of gynogenic embryos was used. For the initiation of the embryos from the cultured ovules, the temperature of 27°C was favoured to that of 24°C. A pronounced and very significant seasonal effect was observed; the month of June was favoured to that of the other summer months tested. The effect of genotype was significant only for certain media. Using the above concentrations and combinations, a yield of viable gynogenic plants (plants/100 cultured ovules) between 6 and 10% (depending on the genotype) was obtained. In each case, 81% of these were haploids.

Root Initiation in Cuttings of Pinus radiata Seedlings II. GROWTH REGULATOR INTERACTIONS

Abstract: Effects of exogenous growth regulators are related to the histological events in adventitious root initiation in hypocotyls of Pinus radiata D. Don. Indolebutyric acid (IBA) is required both for the pre-initiative and post-initiative phases in root development. Kinetin has a marked inhibitory effect over the pre-initiative phase, with escape from inhibition following establishment of meristemoids. Gibberellie acid (GA3) has three distinct effects depending on time of application. While inhibitory during the pre-initiative phase, it strongly enhances rooting if applied at a time coincident with the first observable stage of root initiation. If applied following establishment of meristemoids, GA3 inhibits root formation.

Thidiazuron stimulates shoot regeneration of sugarcane embryogenic callus.

Abstract: Efficient shoot regeneration of sugarcane (Saccharum spp. hybrid cv. CP84-1198) from embryogenic callus cultures has been obtained using thidiazuron (TDZ). Callus was placed on modified Murashige and Skoog (MS) medium containing 2.3 μM 2,4-dichlorophenoxyacetic acid (2,4-D), or 9.3 μM kinetin and 22.3 μM naphthaleneacetic acid (NAA) and compared with the same MS medium supplemented with 0.5, 1.0, 2.5, 5.0 or 10.0 μMTDZ, A11 TDZ treatments resulted in faster shoot regeneration than the kinetin/NAA treatment, and more shoot production than either the 2,4-D or kinetin/NAA treatments. Maximum response, as determined by total number of shoots (26 per explant) and number of shoots greater than 1 cm (4 per explant) 4 wk after initiation, was obtained with 1.0 μM TDZ. The shoots rooted efficiently on MS medium supplemented with 19.7 μM indole-3-butyric acid (IBA). These results indicate that TDZ effectively stimulates sugarcane plant regeneration from embryogenic callus, and may be suitable to use in genetic transformation studies to enhance regeneration of transgenic plants.

Therapeutic potential and mechanism of kinetin as a treatment for the human splicing disease familial dysautonomia

Abstract: Mutations that affect the splicing of pre-mRNA are a major cause of human disease. Familial dysautonomia (FD) is a recessive neurodegenerative disease caused by a T to C transition at base pair 6 of IKBKAP intron 20. This mutation results in variable tissue-specific skipping of exon 20. Previously, we reported that the plant cytokinin kinetin dramatically increases exon 20 inclusion in RNA isolated from cultured FD cells. The goal of the current study was to investigate the nature of the FD splicing defect and the mechanism by which kinetin improves exon inclusion, as such knowledge will facilitate the development of future therapeutics aimed at regulating mRNA splicing. In this study, we demonstrate that treatment of FD lymphoblast cell lines with kinetin increases IKBKAP mRNA and IKAP protein to normal levels. Using a series of minigene constructs, we show that deletion of a region at the end of IKBKAP exon 20 disrupts the ability of kinetin to improve exon inclusion, pinpointing a kinetin responsive sequence element. We next performed a screen of endogenously expressed genes with multiple isoforms resulting from exon skipping events and show that kinetin’s ability to improve exon inclusion is not limited to IKBKAP. Lastly, we highlight the potential of kinetin for the treatment of other human splicing disorders by showing correction of a splicing defect in neurofibromatosis.