Topic
Lambda phage
About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.
Papers published on a yearly basis
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TL;DR: The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA: protein interactions that occur at the initiation of packaging.
Abstract: A mutant of lambda was isolated that grows in the Escherichia coli himA delta/gyrB-him320(Ts) double mutant at 42 degrees C; conditions which are non-permissive for wild-type lambda growth. The responsible mutation, ohm1, alters the 40th codon of the Nul reading frame. The Nul and A gene products comprise the terminase protein which cleaves concatameric DNA into unit-length phage genomes during DNA packaging. The Nul-ohm1 gene product acts in trans to support lambda growth in the double himA/gyrB mutant, and lambda cos154 growth in the single himA mutant. The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA:protein interactions that occur at the initiation of packaging.
37 citations
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TL;DR: Judging both by the relative amounts of deoxyribonucleic acid in the various enteric genomes that are highly homologous and by the conservation of positions of restriction enzyme cleavage sites in these regions, theEnteric genomes have diverged to greater extents in some parts of the genomes than in others.
Abstract: We have assessed the degree of relatedness of several portions of the Escherichia coli genome to the corresponding portions of the genomes of representative enteric bacteria, using the Southern transfer and hybridization technique (E. Southern, J. Mol. Biol. 98:503-517, 1975). The degree of relatedness varied among the regions examined. Judging both by the relative amounts of deoxyribonucleic acid in the various enteric genomes that are highly homologous and by the conservation of positions of restriction enzyme cleavage sites in these regions, the enteric genomes have diverged to greater extents in some parts of the genomes than in others. Portions of the genomes (including the tnaA and thyA genes, the trp operon, and one other unassigned segment) appear to have evolved in concert with the genome as a whole. By contrast, the lacZ gene and portions of the genome that are homologous to phage lambda vary more widely, perhaps reflecting a separate evolutionary origin for these segments of deoxyribonucleic acid.
37 citations
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TL;DR: It is demonstrated that the rpoA341 allele prevents lysogenization of the host strain by bacteriophage lambda, a process dependent upon the action of two phage-encoded activators, and proposed that the inhibition of lysagenization arises from a defective interaction between the phage lambda transcriptional activator CII and the mutant RNA polymerase at the phages promoters pI and pE.
37 citations
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37 citations
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TL;DR: MITOMYCIN C is of biological interest not only because it selectively inhibits cellular deoxyribonucleic acid synthesis1,2 but also in that it induces lambda phage development in Escherichia coli K 123.
Abstract: MITOMYCIN C is of biological interest not only because it selectively inhibits cellular deoxyribonucleic acid synthesis1,2 but also in that it induces lambda phage development in Escherichia coli K 123.
37 citations