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Lambda phage

About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.


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Journal ArticleDOI
01 Jan 1987-Gene
TL;DR: A fast and efficient procedure for generating cDNA libraries in plasmid or phage lambda vectors using oligodeoxynucleotide adapters to insert the cDNA into the vector, avoiding the use of methylating enzymes and subsequent digestion with massive amounts of restriction endonucleases.

24 citations

Journal ArticleDOI
TL;DR: It is shown that the binding of cI protein to OR1 is sufficient to effect an increase in the rate of synthesis of abortive RNA products at PRM, and is readily interpreted in terms of the previously proposed model involving the exclusion of an interfering RNA polymerase from binding at PR.

23 citations

Journal ArticleDOI
TL;DR: Examination of sequence homologies with the gene for rat cytosolic phosphoenolpyruvate carboxykinase indicates a putative control region contained in flanking sequences at the 5' end of the gene.

23 citations

Journal ArticleDOI
TL;DR: The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.
Abstract: Genetic recombination in bacteriophage λ relies on DNA end processing by Exo to expose 3′-tailed strands for annealing and exchange by β protein. Phage λ encodes an additional recombinase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 A. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3′-tailed, or 5′-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.

23 citations

Journal ArticleDOI
TL;DR: Since bar-containing RNA has the properties of a messenger, it is proposed that its translation leads to drop-off and accumulation of peptidyl-tRNA in pth-defective cells.

23 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20226
20219
20209
20195
20188
20177