scispace - formally typeset
Search or ask a question
Topic

Lambda phage

About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.


Papers
More filters
Journal ArticleDOI
TL;DR: A recognition sequence for the K-restriction system has been localized within the trpE gene of Escherichia coli by selecting for phage mutants which have simultaneously lost two restriction-recognition sites.

19 citations

Journal ArticleDOI
TL;DR: The denV gene of bacteriophage T4 has been cloned into Escherichia coli K-12 by inserting appropriate fragments of cytosine-containing T4 DNA into the Sal I site of the plasmid pBR322, which results in enhanced UV resistance that is more pronounced in stationary- than in exponential-phase cultures.
Abstract: The denV gene of bacteriophage T4 has been cloned into Escherichia coli K-12 by inserting appropriate fragments of cytosine-containing T4 DNA into the Sal I site of the plasmid pBR322. The denV gene codes for an enzyme that initiates the excision repair of pyrimidine dimers produced in DNA by UV. In uvrA recA mutants, deficient in an early step in excision repair, the cloned DNA results in enhanced UV resistance that is more pronounced in stationary- than in exponential-phase cultures. The expression of the cloned DNA also results in the enhanced survival of UV-irradiated phage lambda or of a denV mutant of phage T4 and in removal of dimers from the DNA of UV-irradiated cells.

19 citations

Journal ArticleDOI
TL;DR: The results suggest that certain functional proteins engineered by computer design between human fibronectin cell binding domain and lambda phage J protein can be produced.

19 citations

Journal ArticleDOI
01 Feb 1989-Virology
TL;DR: Results indicate that IHF may play a significant auxiliary role in repressing transcription in the prophage state and that plasmids carrying the attP fragment cannot be established and maintained in IHF-hosts.

19 citations

Journal ArticleDOI
TL;DR: Using molecular cloning techniques, the BL/Ka(B) virus was derived from an endogenous N-tropic virus closely related to Akv by recombinational events which altered the sequence in the last half of the gag gene and the first third of the pol gene, probably responsible for the observed difference in the Fv-1 tropism of the two viruses.
Abstract: Using molecular cloning techniques, we amplified the unintegrated, linear proviral DNA of the BL/Ka(B) virus, a non-leukemogenic retrovirus of mouse strain C57BL/Ka. Two independent clones in lambda phage vector 607 and one subclone in pBR322 were infective when transfected into mouse fibroblasts. Analysis of the progeny virus revealed biological properties and a restriction map identical to those of the parental viral shock. Comparison of the restriction map with the maps of other ecotropic murine viruses reveals many similarities. Particularly interesting is the comparison of the N-tropic Akv virus and the B-tropic BL/Ka(B) virus. The long terminal repeats of the two viruses are virtually identical, as are 22 of 23 restriction sites located outside of the region which spans from 1.8 to 3.8 kilobases from the left end of the genome. Within this region, however, only three of nine sites examined are shared. This suggests that the BL/Ka(B) virus was derived from an endogenous N-tropic virus closely related to Akv by recombinational events which altered the sequence in the last half of the gag gene and the first third of the pol gene. This change is probably responsible for the observed difference in the Fv-1 tropism of the two viruses.

19 citations


Network Information
Related Topics (5)
Mutant
74.5K papers, 3.4M citations
88% related
Transcription (biology)
56.5K papers, 2.9M citations
87% related
Peptide sequence
84.1K papers, 4.3M citations
87% related
RNA
111.6K papers, 5.4M citations
85% related
DNA
107.1K papers, 4.7M citations
84% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20226
20219
20209
20195
20188
20177