Topic
Lambda phage
About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.
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TL;DR: Two new lambda vectors were constructed which permit cloning of genes that are potentially lethal if cloned in analogous plasmid vectors, and two plasmids, pPR110 and pPR111, were constructed from lambda DL10 and lambda DL11 respectively, and pCQV2.
16 citations
01 Jan 2012
TL;DR: In this chapter, recent advances in the understanding of mechanisms of bacteriophage l development are summarized and discussed and studies on phage DNA injection, prophage maintenance and induction, and lysis of the host cell will be presented.
Abstract: Bacteriophage l, rediscovered in the early 1950s, has served as a model in molecular biology studies for decades. Although currently more complex organisms and more complicated biological systems can be studied, this phage is still an excellent model to investigate principles of biological processes occurring at the molecular level. In fact, very few other biological models provide possibilities to examine regulations of biological mechanisms as detailed as performed with l. In this chapter, recent advances in our understanding of mechanisms of bacteriophage l development are summarized and discussed. Particularly, studies on (i) phage DNA
injection, (ii) molecular bases of the lysis-versus-lysogenization decision and the lysogenization process itself, (iii) prophage maintenance and induction, (iv), l DNA replication, (v) phage-encoded recombination systems, (vi) transcription antitermination, (vii) formation
of the virion structure, and (viii) lysis of the host cell, as published during several past years, will be presented.
16 citations
TL;DR: DNA of the specialized transducing phage lambda dapB2 has been digested with the restriction endonucleases Bam I, HindIII, or both together to generate fragments originating from the bacterial substitution on the phage to be identified by coupled transcription and translation of plasmid DNA templates in vitro or by the stimulation of protein synthesis promoted by these plasmids in an ultraviolet irradiated host.
16 citations
TL;DR: The strong regulated expression cassette with the Pr promoter of lambda phage and the cooperative expression of barnase and barstar under its control have been applied to expression of these proteins in Escherichia coli.
16 citations
TL;DR: In this article, two mutations of the phage attachment site were characterized for integrative but not excisive recombination, and they produced the same base change in the P'3 binding site for Int protein 79 base-pairs from the center of the crossover region for site-specific recombination.
16 citations