Topic
Lambda phage
About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.
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TL;DR: The results support the notion that there are multiple pathways for ultraviolet radiation-induced mutagenesis, including direct and direct mutageneses in λ induced by ultraviolet radication, which were dissimilar in dependence on recA+, lex+, polA+ and certain other genes relevant to DNA repair.
79 citations
TL;DR: In this article, the phase variation control region is carried on the specialized transducing phage, which is not present on the phage but rather must be supplied by the recipient strain in the transduction.
Abstract: Expression of type 1 fimbriae in Escherichia coli exhibits phase variation, whereby individual cells can alternate between states of organelle expression (Fim+) and nonexpression (Fim-). Strains with a fimD-lac operon fusion, in which lac, rather than fimD, expression is under the control of the fimD promoter, undergo Lac+ in equilibrium Lac- phase variation, instead. After positioning a lambda prophage adjacent to the operon fusion, we were able to isolate specialized lambda phage carrying both the fimD-lac fusion and the phase variation control region. Introduction of such phage into an Fim+ strain resulted in construction of a strain with a double, independently switching phenotype (Fim+ in equilibrium Fim- and Lac+ in equilibrium Lac-), demonstrating that the region controlling phase variation is contiguous with the fimD-lac operon fusion and is cis acting. When the specialized lambda phage was propagated on a delta lac delta fim strain, phase variation occurred within the plaques, confirming that the phase variation control region is carried on the specialized transducing phage. All lysogens acquired the Lac+ in equilibrium Lac- phenotype, except for two nonswitching Lac+ recombinants, which acquired Lac+ in equilibrium Lac- phase variation only by trans complementation with fim. Phase variation of type 1 fimbriae, therefore, appears to involve both a cis-active element, which is cloned on a specialized lambda phage, and a trans-active permissive factor, which is not present on the phage, but rather must be supplied by the recipient strain in the transduction.
79 citations
TL;DR: It is concluded that the pel − mutant allows adsorption of λ but that it inhibits the subsequent injection of the phage DNA.
78 citations
TL;DR: The isogenic pair of phage developed in this study consisting of the long-circulating marker-rescued lambdaArgo phage, and the parental wild type phage can be used for studies of viral recognition mechanisms of the innate immune system and for the development of more effective antibacterial therapeutic phage strains.
78 citations
TL;DR: It is demonstrated that none of the these nus mutations affects the synthesis of N or the other nus gene products to cause a defect in antitermination, and rather, these mutations have inactivated a set of specific host components, the Nus factors, which are essential for N activity.
78 citations