Topic
Lambda phage
About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.
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TL;DR: A system for construction of E. coli strains with multiple DNA insertions in the chromosome, based on elements of modules for site specific recombination of Tn1545 and phage lambda, has been developed and shows the utility of the system.
60 citations
TL;DR: This work aims to demonstrate the importance of knowing the carrier and removal status of canine coronavirus, as a source of infection for other animals, not necessarily belonging to the same breeds.
Abstract: S.A. Wilcox (corresponding author) is at the Murdoch Institute, Royal Children's Hospital, Melbourne 3052, Australia. Tel: (+61) 03 9345 5045; Fax: (+61) 03 9348 1391. R. Toder is at the School of Genetics and Human Variation, La Trobe University, Melbourne 3083, Australia. Tel: ( § 61) 03 9479 2770; Fax: ( + 61) 03 9479 2480; Emaih gensaw@lure.latrobe.edu.au. J. W. Foster is at the Department of Genetics, Cambridge University, Cambridge CB2 3EH, UK. Tel: ( + 44) 223 333988; Fax: (+ 44) 223 333992.
60 citations
TL;DR: It is shown that a large part, though not all, of the effect of lambda infection on host protein synthesis can be ascribed to the early region that contains phage genes Ea10 and ral, and that shorter deletions in this region gave intermediate effects.
Abstract: We used two-dimensional gel electrophoresis to quantitate the changes in rates of synthesis that follow phage lambda infection for 21 Escherichia coli proteins, including groE and dnaK proteins. Although total protein synthesis and the rates of synthesis of most individual E. coli proteins decreased after infection, some proteins, including groE protein, dnaK protein, and stringent starvation protein, showed increases to rates substantially above their preinfection rates. Infection by lambda Q- affected host synthesis in the same way as infection by gamma+, whereas infection by lambda N- showed no detectable effect on host synthesis. Deletion of the early genes between att and N abolished the effect, and shorter deletions in this region gave intermediate effects. By this sort of deletion mapping, we show that a large part, though not all, of the effect of lambda infection on host protein synthesis can be ascribed to the early region that contains phage genes Ea10 and ral. We compared the changes in protein synthesis after infection with the changes that occur in uninfected cells upon heat shock or amino acid starvation. The spectrum of changes that occurred on infection was very different from that seen after heat shock but quite similar to that seen during amino acid starvation. Despite this similarity of the effects of lambda infection and starvation, we did not detect any increase in the level of guanosine tetraphosphate during infection. We show that the groE protein is the same protein as B56.5 of Lemaux et al. (Cell 13:427-434, 1978) and A protein of Subramanian et al. (Eur. J. Biochem. 67:591-601, 1976).
60 citations
TL;DR: Observations and database searches demonstrate sequence similarity between proteins of phage from gram-positive bacteria, proteobacteria, and Archaea, constrain models ofphage evolution.
60 citations
TL;DR: Two mutant Int proteins are shown to be proficient to perform intramolecular integrative and excisive recombination in co-transfection assays inside human cells, implying that eukaryotic co-factors, which could functionally replace the prokaryotic ones normally required for wild-type Int, are most likely not present in human cells.
60 citations