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Lambda phage

About: Lambda phage is a research topic. Over the lifetime, 1609 publications have been published within this topic receiving 84675 citations. The topic is also known as: Enterobacteria phage lambda.


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Journal ArticleDOI
TL;DR: It is demonstrated that cII in solution is a tetrameric protein and that it undergoes specific processing at its NH2-terminal end and is characterized as to its molar extinction coefficient, molecular weight, amino acid composition, isoelectric point, alpha-helical content, and antigenic capability.

52 citations

Journal ArticleDOI
TL;DR: The result indicates that a trans -active product of tdel 33 is responsible for its high frequency of duplication production, and a new mutant of Escherichia coli which does not plate λ deletion phages is made use.

52 citations

Journal ArticleDOI
01 Nov 1971-Virology
TL;DR: It is proposed that the λ Spi+ products convert recA−recB+ bacteria to RecB− phenocopies, as though the Spi- products could convert a recB+ host to a RecB+ phenocopy.

51 citations

Journal ArticleDOI
TL;DR: The recombinase from native phage or prophage can significantly promote the genetic recombineering frequency in its host, providing an effective genetic tool for constructing genetically engineered strains and investigating bacterial physiology.
Abstract: Bacillus subtilis and its closely related species are important strains for industry, agriculture, and medicine However, it is difficult to perform genetic manipulations using the endogenous recombination machinery In many bacteria, phage recombineering systems have been employed to improve recombineering frequencies To date, an efficient phage recombineering system for B subtilis has not been reported Here, we, for the first time, identified that GP35 from the native phage SPP1 exhibited a high recombination activity in B subtilis On this basis, we developed a high-efficiency GP35-meditated recombineering system Taking single-stranded DNA (ssDNA) as a recombineering substrate, ten recombinases from diverse sources were investigated in B subtilis W168 GP35 showed the highest recombineering frequency (171 ± 015 × 10−1) Besides targeting the purine nucleoside phosphorylase gene (deoD), we also demonstrated the utility of GP35 and Beta from Escherichia coli lambda phage by deleting the alpha-amylase gene (amyE) and uracil phosphoribosyltransferase gene (upp) In all three genetic loci, GP35 exhibited a higher frequency than Beta Moreover, a phylogenetic tree comparing the kinship of different recombinase hosts with B subtilis was constructed, and the relationship between the recombineering frequency and the kinship of the host was further analyzed The results suggested that closer kinship to B subtilis resulted in higher frequency in B subtilis In conclusion, the recombinase from native phage or prophage can significantly promote the genetic recombineering frequency in its host, providing an effective genetic tool for constructing genetically engineered strains and investigating bacterial physiology

51 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20226
20219
20209
20195
20188
20177