Showing papers on "Lanosterol published in 1967"
TL;DR: Evidence for the role of Es1 and Es2 as intermediates in ergosterol biosynthesis is discussed, and it is suggested that a C27 sterol is the acceptor in the transmethylation reaction.
100 citations
TL;DR: It has been shown that this transformation, hitherto considered to take place through the agency of one enzyme ("squalene oxidocyclase-1"), actually involves the formation of squalene-2,3-oxide as an intermediate.
78 citations
TL;DR: An enzyme catalyzing the anaerobic cyclization of 2,3-oxidosqualene into lanosterol has been extracted from hog liver microsomes by treatment with deoxycholate and produced a soluble 20-fold purified cyclase.
70 citations
TL;DR: A cell-free system capable of introducing 14C from methyl-labeled methionine into lanosterol, desmosterol, and cycloartenol was obtained in a supernatant from the centrifugation, confirming the earlier prediction that the Δ24(25) bond is necessary in order that alkylation may proceed.
44 citations
TL;DR: On the basis of unambiguous degradations it is shown that the C-alkylation step in ergosterol biosynthesis is accompanied by the migration of a hydrogen atom from C-24 to C-25.
Abstract: 1. A convenient synthesis of 3-hydroxytrisnorlanost-8-en-24-al and its conversion into [24-3H]lanosterol and [26,27-14C2]lanosterol is described. 2. A method for the efficient incorporation of lanosterol into ergosterol by the whole cells of Saccharomyces cerevisiae is also described. 3. It is shown that in the biosynthesis of ergosterol from doubly labelled lanosterol the C-24 hydrogen atom of lanosterol is retained in ergosterol. 4. On the basis of unambiguous degradations it is shown that the C-alkylation step in ergosterol biosynthesis is accompanied by the migration of a hydrogen atom from C-24 to C-25. 5. The mechanism for the biosynthesis of the ergosterol side chain is presented. 6. Mechanisms of other C-alkylation reactions are also discussed.
34 citations
TL;DR: C1 transfer to the Δ24 bond of 8,24-lanostadien-3β-ol (lanosterol) or of 9,19-cyclo-24- Lanosten-3 β-ols (cycloartenol) was inhibited by triparanol when either of the two steroids was incubated with methionine as the C1 source in a cell-free system from germinating peas.
32 citations
27 citations
TL;DR: The incorporation of mevalonic acid-2- 14 C into cut flower stalks of Taraxacum officinale L, whose principal sterol and triterpene respectively in the dried form is β-sitosterol and β-amyrin, was studied at intervals of 0·5 hr to 2 weeks as discussed by the authors.
20 citations
TL;DR: The cyclic triterpenes lanosterol, 24-methylenecycloartanol, euphol and α-amyrin have been shown to partially inhibit the incorporation of L-mevalonate-2-14C into the total non-saponifiable lipid and the digitonin-precipitable material of rat liver homogenates.
6 citations