Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
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TL;DR: It is demonstrated that OE of the homologous LS gene can enhance lanosterol accumulation and a large precursor supply promotes GA biosynthesis.
24 citations
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TL;DR: In this article, the sterols 24,25-dihydro-4α-methyl-24-methylene-lanosterol and 3β-hydroxy 4β-methylcholesta-8(14),24-diene have been isolated from yeast residues and their structures have been determined.
Abstract: 24,25-Dihydro-24-methylenelanosterol has been synthesised from lanosterol and investigated as a potential precursor of eburicoic acid and of ergosterol.Two new sterols, 24,25-dihydro-4α-methyl-24-methylenezymosterol and 3β-hydroxy-4α-methylcholesta-8(14),24-diene have been isolated from yeast residues and their structures have been determined. The constitution of 24,25-dihydro-4α-methyl-24-methylenezymosterol has been confirmed by a partial synthesis from the more abundant 4α-methylzymosterol.4α-Methylzymosterol, 24,25-dihydro-4α-methyl-24-methylenezymosterol and obtusifoliol have been shown to be incorporated into ergosterol in yeast cultures. 4,4-Dimethylergosterol is, however, not a precursor.By a trapping experiment in which tritium-labelled squalene oxide and unlabelled 24,25-dihydro-24-methylene-lanosterol were used it has been shown that the latter is almost certainly not on the biogenetically preferred route from lanosterol to ergosterol.
23 citations
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TL;DR: In this article, a study was made of the methyl sterols involved in sterol biosynthesis in developing rat brain and the determination of the endogenous sterols present in 15-day-old rat brain was conducted to establish which of these sterols could be labeled by a minced tissue preparation in the presence of [2-14C]mevalonic acid.
23 citations
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TL;DR: Investigation of the metabolism of exogenously provided delta24-sterols by whole cell cultures of a polyene-resistant mutant of Candida albicans blocked at removal of the C-14 methyl group indicated that zymosterol was a better (40 X) substrate than endogenous lanosterol.
Abstract: We have investigated the metabolism of exogenously provided Δ24-sterols by whole cell cultures of a polyene-resistant mutant (D10) of Candida albicans blocked at removal of the C-14 methyl group. Comparison of the relative efficiencies of transmethylation at C-24 of selected sterol substrates revealed the following substrate preferences of the Candida Δ24-sterol methyltransferase (EC 2.1.1.41): zymosterol > 4α-methylzymosterol > 14α-methylzymosterol. Exogenous 4,4-dimethylzymosterol was not transmethylated by mutant D10. Incorporation of the 14C-labelled methyl group of S-adenosyl-L-[methyl-14C]methionine into the sterols of a D10 culture preloaded with zymosterol indicated that zymosterol was a better (40 ×) substrate than endogenous lanosterol. Feeding zymosterol to D10 and a polyene-resistant strain of Saccharomyces cerevisiae (Nys-P100) that was also blocked at removal of the C-14 methyl group gave 24-methyl sterols possessing Δ22 and ring B unsaturation. Mutant D10 was able to produce ergosterol from...
23 citations
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TL;DR: The 4,4-dimethyl- and the 4-desmethylsterols were in the configurational series with a 20α-H-atom and are the probable intermediates from cycloartenol to cholesterol and 24-alkylcholesterol, respectively.
23 citations