Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
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TL;DR: Conditions have been established for the incorporation of labeled acetate and mevalonate into sterols of rat testicular tissue and labelled acetate was incorporated into all fractions of testicular sterols in vivo.
16 citations
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TL;DR: An altered cytochrome P-450 was partially purified from Saccharomyces cerevisiae mutant SG1 which is defective in lanosterol 14 alpha-demethylation, suggesting the presence of a nitrogenous ligand such as histidine of the apoprotein at the 6th coordination position.
16 citations
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TL;DR: Results of this study suggest that initial steps of steroid hormone production in murine Leydig cells are mostly dependent on exogenously derived cholesterol, while from day 13.5 onwards, mouse Leydigs cells are able to synthesize cholesterol and are therefore notdependent on exogenous cholesterol resources.
Abstract: Sexual differentiation is a carefully regulated process that ultimately results in a development of the male or female phenotype. Proper development of the male phenotype is dependent upon the action of testosterone, produced by Leydig cells, and antimullerian hormone, produced by Sertoli cells. Leydig cells start to produce testosterone around day 12.5 in the fetal mouse testis, and continue to produce high levels of testosterone throughout the gestation. In the present study we examined whether expression of two enzymes, lanosterol 14α -demethylase (cyp51) and cytochrome P450 NADPH reductase, involved in the cholesterol production, occurs simultaneously with proteins required for the production of steroid hormones. Immunocytochemical staining with antibodies against lanosterol 14α -demethylase , cytochrome P450 NADPH reductase, steroidogenic acute regulatory protein (StAR) and 3beta-hydroxysteroid dehydrogenase I (3β -HSD I) was used to determine the ontogeny of expression of these four proteins. As expected, 3β -HSD I and StAR proteins were detected on day 12.5 p.c., while expression of cyp51 and NADPH cytochrome P450 reductase appeared one day later, on day 13.5. Thereafter, the expression of all four proteins remained strong throughout the gestation. In human fetal testes, all four proteins were expressed in the Leydig cells at all ages studied, although very young samples from the time of initial Leydig cell development were not examined as they were not available. Results of this study suggest that initial steps of steroid hormone production in murine Leydig cells are dependent on exogenously derived cholesterol. Later on (from day 13.5 onwards), murine and human Leydig cells are able to synthesize cholesterol and are therefore not dependent on exogenous cholesterol resources.
16 citations
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TL;DR: (S)-Squalene 2,3-epoxide was found to be the exclusive precursor of lanosterol in pig liver, Lanosterol, and ergosterols in yeast, and β-amyrin, lupeol and cycloartenol in pea seedlings.
Abstract: (S)-Squalene 2,3-epoxide was found to be the exclusive precursor of lanosterol in pig liver, lanosterol, and ergosterol in yeast (Saccharamyces cerevisiae), and β-amyrin, lupeol and cycloartenol in pea seedlings.
16 citations
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TL;DR: A N,N-dimethylaminopropyl derivative showed promising inhibition of Trypanosoma cruzi OSC in combination with low cytotoxicity, and showed significant reduction of cholesterol biosynthesis in a human cell line.
16 citations