Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
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TL;DR: Measurement of isotope ratios in 5α-stigmast-9(11)-en_ 3β -ol (I), a C 29 -sterol, biosynthesised in Costus speciosus from 3RS-mevalonate -[2- 14 C,4R- 3 H] have shown that squalene is possibly metabolised into I via Δ 9(11) lanosterol (VII) rather than Δ 8(9) lanost
9 citations
TL;DR: It was demonstrated that nearly all the sterol radioactivity could be accounted for by 4,4,14α-trimethyl-5α-cholesta-8,24-dien-3β-ol (lanosterol) and three sterols, which were slightly more hydrophilic than cholesterol.
9 citations
TL;DR: CYP51–FprD fusion enzymes showed distinct spectral properties of both flavoprotein and CYP, and were fused together with designed linker sequences to construct an artificial self‐sufficient cytochrome P450 monooxygenase (CYP) with only FprD, CYP51, and iron–sulfur containing FPRD.
Abstract: CYP51, a sterol 14α-demethylase, is one of the key enzymes involved in sterol biosynthesis and requires electrons transferred from its redox partners. A unique CYP51 from Nocardia farcinica IFM10152 forms a distinct cluster with iron-sulfur containing NADPH-P450 reductase (FprD) downstream of CYP51. Previously, sequence alignment of nine reductases from N. farcinica revealed that FprC, FprD, and FprH have an additional sequence at their N-termini that has very high identity with iron-sulfur clustered ferredoxin G (FdxG). To construct an artificial self-sufficient cytochrome P450 monooxygenase (CYP) with only FprD, CYP51, and iron-sulfur containing FprD were fused together with designed linker sequences. CYP51-FprD fusion enzymes showed distinct spectral properties of both flavoprotein and CYP. CYP51-FprD F1 and F2 in recombinant Escherichia coli BL21(DE3) catalyzed demethylation of lanosterol more efficiently, with k(cat) /K(m) values of 96.91 and 105.79 nmol/min/nmol, respectively, which are about 35-fold higher compared to those of CYP51 and FprD alone.
9 citations
TL;DR: It is shown unequivocally that the compounds thus obtained have the unnatural 14β-configuration.
Abstract: Methods are described for the removal of carbon atom 32 from certain 32-functionalised lanosterol derivatives. It is shown unequivocally that the compounds thus obtained have the unnatural 14β-configuration.
9 citations
TL;DR: Cholesterol biosynthesis in cultured human skin fibroblasts was studied by [14C]acetic acid incorporation into non-saponifiable lipids and quantification of labeled cholesterol among its precursors, indicating the existence of a relationship between endogenous cholesterol synthesis and cell growth and sheds additional light on the role of post-HMG-CoA regulation in this phenomenon.
9 citations