Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
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TL;DR: Direct evolution was used to find cycloartenol synthase residues that affect cyclopropyl ring formation, selecting randomly generated cycloarternal synthase mutants for their ability to genetically complement a yeast strain lacking lanosterol synth enzyme.
67 citations
TL;DR: Interestingly, platelets can convert [14C]desmosterol to cholesterol, although not nearly as efficiently as liver, which suggests that a major block exists in vitro in demethylation of lanosterol by human platelets.
67 citations
TL;DR: The azole antifungals ketoconazole and itraconazole possess in vitro antileishmanial activity against Leishmania mexicana mexicanana amastigotes in macrophages and all [14C]acetate was incorporated in ergosterol, suggesting an inhibition in cholesterol synthesis in the host cells.
66 citations
TL;DR: Lanosterol and related CzO sterols occur in considerable quantity in wool fat but have not so far been isolated from the principal sites of sterol biogenesis in the animal body.
66 citations
TL;DR: It is concluded that AtSAT1 mediates phytosterol ester biosynthesis, alternative to the route previously described for phospholipid:sterol acyltransferase, and provides the molecular basis for modification of phytosterone ester level in seeds.
Abstract: Fatty acyl esters of phytosterols are a major form of sterol conjugates distributed in many parts of plants. In this study we report an Arabidopsis (Arabidopsis thaliana) gene, AtSAT1 (At3g51970), which encodes for a novel sterol O-acyltransferase. When expressed in yeast (Saccharomyces cerevisiae), AtSAT1 mediated production of sterol esters enriched with lanosterol. Enzyme property assessment using cell-free lysate of yeast expressing AtSAT1 suggested the enzyme preferred cycloartenol as acyl acceptor and saturated fatty acyl-Coenyzme A as acyl donor. Taking a transgenic approach, we showed that Arabidopsis seeds overexpressing AtSAT1 accumulated fatty acyl esters of cycloartenol, accompanied by substantial decreases in ester content of campesterol and β-sitosterol. Furthermore, fatty acid components of sterol esters from the transgenic lines were enriched with saturated and long-chain fatty acids. The enhanced AtSAT1 expression resulted in decreased level of free sterols, but the total sterol content in the transgenic seeds increased by up to 60% compared to that in wild type. We conclude that AtSAT1 mediates phytosterol ester biosynthesis, alternative to the route previously described for phospholipid:sterol acyltransferase, and provides the molecular basis for modification of phytosterol ester level in seeds.
66 citations