Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
Papers published on a yearly basis
Papers
More filters
TL;DR: Inhibitors of P. carinii viability as determined by quantitation of cellular ATP levels in a population of organisms varied in inhibitory effect; the most effective included drugs targeted at squalene synthase,Squalene epoxide-lanosterol cyclase, and Δ8 to Δ7isomerase.
Abstract: Pneumocystis carinii synthesizes sterols with a double bond at C-7 of the sterol nucleus and an alkyl group with one or two carbons at C-24 of the side chain. Also, some human-derived Pneumocystis carinii f. sp. hominis strains contain lanosterol derivatives with an alkyl group at C-24. These unique sterols have not been found in other pathogens of mammalian lungs. Thus, P. carinii may have important differences in its susceptibility to drugs known to block reactions in ergosterol biosynthesis in other fungi. In the present study, inhibitors of 3-hydroxy-3-methyglutaryl coenzyme A reductase, squalene synthase, squalene epoxidase, squalene epoxide-lanosterol cyclase, lanosterol demethylase, Delta(8) to Delta(7) isomerase, and S-adenosylmethionine:sterol methyltransferase were tested for their effects on P. carinii viability as determined by quantitation of cellular ATP levels in a population of organisms. Compounds within each category varied in inhibitory effect; the most effective included drugs targeted at squalene synthase, squalene epoxide-lanosterol cyclase, and Delta(8) to Delta(7) isomerase. Some drugs that are potent against ergosterol-synthesizing fungi had little effect against P. carinii, suggesting that substrates and/or enzymes in P. carinii sterol biosynthetic reactions are distinct. Amphotericin B is ineffective in clearing P. carinii infections at clinical doses; however, this drug apparently binds to sterols and causes permeability changes in P. carinii membranes, since it reduced cellular ATP levels in a dose-dependent fashion.
45 citations
TL;DR: Monocycles comprise most of the Gly mutant product, showing that an alternate cyclization route can be made the major pathway by a single nonpolar mutation.
45 citations
TL;DR: In this paper, Lanost-8-ene-3β,32-diol is synthesized and is shown to be demethylated by a rat liver microsomal preparation to give 4,4′-dimethylcholesta-8,14-dien-3 β-ol with the release of C-32 as formic acid.
45 citations
TL;DR: The premise that oxylanosterols regulate HMG-CoA reductase expression through a post-transcriptional process which may be distinct from other previously described sterol regulatory mechanisms is supported.
44 citations
TL;DR: Normal human lymphocytes stimulated by culture in a lipid-depleted medium were used to study the post-HMG CoA regulations of cholesterol biosynthesis and an intermediate which accumulates in the cell has inhibitory properties towards H MG CoA reductase and thus could be considered as an endogenous cellular bioregulator ofolesterol biosynthesis.
44 citations