Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
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TL;DR: A series of phospholipids, including previously undescribed compounds 4-7, were isolated by a bioactivity-guided fractionation from the marine sponge Spirastrella abata as inhibitors of cholesterol biosynthesis in human liver cells.
Abstract: A series of phospholipids, including previously undescribed compounds 4-7, were isolated by a bioactivity-guided fractionation from the marine sponge Spirastrella abata as inhibitors of cholesterol biosynthesis in human liver cells. These compounds were identified as lyso-PAF analogues (1-5) and lysophosphatidylcholines (6, 7) based on NMR and MS analyses. Compounds 1-7 specifically blocked the conversion of lanosterol into cholesterol in the Chang liver cell.
35 citations
TL;DR: Homogenates of human ovaries obtained from fetuses at 20 and 22 weeks of gestational age were incubated with sodium acetate-l-14C and small, but definite quantities of radioactive pregnenolone and progesterone were identified.
Abstract: Homogenates of human ovaries obtained from fetuses at 20 and 22 weeks of gestational age were incubated with sodium acetate-l-14C. The extracts were analyzed for sterols and steroids by the reverse isotope dilution technique. Purification and identification of the radioactive metabolites were achieved by paper and thin-layer chromatography, derivative formation and crystallization to constant specific activity. Evidence has been obtained for the formation of lanosterol and cholesterol. Small, but definite quantities of radioactive pregnenolone and progesterone were identified. No evidence was found for the formation of dehydroepiandrosterone, androstenedione, testosterone and estrogens.
35 citations
TL;DR: A mutant strain of Saccharomyces cerevisiae which requires only a sterol for growth has been isolated and Sterol analysis suggests a block in ergosterol formation at the level of conversion of squalene to lanosterol.
35 citations
TL;DR: Results provide the first evidence in support of a previously unknown regulatory role for the 14-reductase in the overall cholesterol synthetic pathway and suggest that the solubilized enzyme is the principal 14- reductase of microsomes.
35 citations
TL;DR: It is reported that SM is stabilized by unsaturated fatty acids, and the mechanism of oleate-mediated stabilization appeared to occur through reduced ubiquitination by the E3 ubiquitin ligase MARCH6.
Abstract: SM (squalene mono-oxygenase) catalyses the first oxygenation step in cholesterol synthesis, immediately before the formation of the steroid backbone at lanosterol. SM is an important control point in the pathway, and is regulated at the post-translational level by accelerated cholesterol-dependent ubiquitination and proteasomal degradation, which is associated with the accumulation of squalene. Using model cell systems, we report that SM is stabilized by unsaturated fatty acids. Treatment with unsaturated fatty acids such as oleate, but not saturated fatty acids, increased protein levels of SM or SM-N100–GFP (the first 100 amino acids of SM fused to GFP) at the post-translational level and partially overcame cholesterol-dependent degradation, as well as reversing cholesterol-dependent squalene accumulation. Maximum stabilization required activation of fatty acids, but not triacylglycerol or phosphatidylcholine synthesis. The mechanism of oleate-mediated stabilization appeared to occur through reduced ubiquitination by the E3 ubiquitin ligase MARCH6. Stabilization of a cholesterol biosynthetic enzyme by unsaturated fatty acids may help maintain a constant cholesterol/phospholipid ratio.
35 citations