Lanosterol

TL;DR: It is predicted that orientation of the hydrophobic tert-butyl and p-chlorophenyl groups relative to the azole nitrogen is important to fit the antifungal agent in the active site of the demethylase.

TL;DR: It is concluded from measurements of the 3H:14C ratios found in these various compounds that the 4α-methyl group of lanosterol is removed before the 4β- methyl group and that the 2,4,14α-trimethyl-5α-cholesta-8,24-dien-3β-ol originates as the 4 β-methyl Group of Lanosterol.

TL;DR: It is reported that sterol intermediates in cholesterol synthesis, beginning with the initial post-cyclization sterol, lanosterol, continuing with zy mosterol, and ending with desmosterol are also substrates for CYP27A1, and the retention times and major mass fragments of these novel metabolites are characterized.

TL;DR: The metabolism of Delta(7)-cholestenol, cholesterol, and cholestanol was examined in a patient with cerebrotendinous xanthomatosis after intravenous pulse-labeling with a mixture of dl-[2-(14)C]mevalonate and stereospecific 3S,4S,3R,4R-[4-(3)H]meValonate], showing that sterol synthesis proceeded in the following sequence.

TL;DR: This previously unrecognized catalytic competence for an enzyme of sterol biosynthesis, together with phylogenomic analyses, suggest that mutational divergence of a promiscuous SMT produced substrate- and phyla-specific SMT1 and SMT2 isoforms in red and green algae.