Topic
Lanosterol
About: Lanosterol is a research topic. Over the lifetime, 1239 publications have been published within this topic receiving 36737 citations. The topic is also known as: (3β)-lanosta-8,24-dien-3-ol & (3β,20R)-lanosta-8,24-dien-3-ol.
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TL;DR: A bicyclic intermediate with two altered deprotonation products, in conjunction with lanosterol, were isolated from the ERG7(Y707X) mutants, indicating that the Tyr707 residue may play a functional role in stabilizing the chair-boat bicyclic C-8 cation and the lanosteryl C- 8/C-9 cation intermediates.
28 citations
27 citations
TL;DR: Squalene-2,3-epoxide: lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein and showed a single band on SDS-polyacrylamide gel electrophoresis with a molecular weight of 75 kD.
Abstract: Squalene-2, 3-epoxide : lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein. The purified enzyme showed a single band on SDS-polyacrylamide gel electrophoresis with a molecular weight of 75 kD. In its native state it behaved as a homo-dimer. The isoelectric point of 5.5 and the apparent Km value for (&3S)-squalene-epoxide of 55 μM were estimated for the cyclase.
27 citations
TL;DR: These compounds were efficiently incorporated by cultures of Ochromonas malhamensis into poriferasterol, the major sterol of this organism, and the significance of these results is discussed in relation to phytosterol biosynthesis.
27 citations
TL;DR: To study the involvement of 93-1 9-cyclopropane sterols in phytosterol biosynthesis, the conversion of 24-methylene, cycloartenol, and [2-3H2]lanosterol into poriferasterol by the phytoflagellate Ochromonas malhamens8s is examined.
Abstract: rence of cycloartenol (I) and 24-methylenecycloartanol (III) in plants and their rapid labelling from [2-14C]mevalonate and [1-14C]acetate under conditions where phytosterols are being actively synthesized (see references above). To study the involvement of 93-1 9-cyclopropane sterols in phytosterol biosynthesis we have now examined the conversion of 24-methylene[2-3H2]cycloartanol, [2 3H2]cycloartenol, [2 3H2]Cycloartenone and [2-3H2]lanosterol into poriferasterol (IV) by the phytoflagellate Ochromonas malhamens8s. Experimental and results. Lanosterol, cycloartenol and 24-methylenecycloartanol, purified by t.l.c. on AgNO3-impregnated silica gel, were converted into the corresponding 3-ketones by chromic acid oxidation and then labelled with 3H at C-2 by exchange on a basic alumina column that
27 citations