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Lead acetate

About: Lead acetate is a research topic. Over the lifetime, 2636 publications have been published within this topic receiving 69739 citations.


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Journal ArticleDOI
TL;DR: The use of lead acetate as a vital stain adds another method for the study of periodic appositional patterns of hard tissues and is conducive to quantitative measurements.
Abstract: The use of lead acetate as a vital stain adds another method for the study of periodic appositional patterns of hard tissues. The distinct advantage of this technique is that these tissues can be decalcified and examined histologically without loss of the marker. The following method was used in preparation of the sections shown in the text. The rats received an intravenous injection of 4 mg lead acetate/kg body weight. After sacrifice, the tissues were decalcified in 1% HCL through which H2S was constantly bubbled. The action of the H2S gas is to convert the lead at sites of calcification to insoluble lead sulfide. Upon completion of decalcification the sections were embedded in 30% gelatin, and frozen sections at 15–20 μ were cut. The sections were then placed in a 0.1% solution of gold chloride for ten minutes. Next, they were placed in a 5% solution of sodium bisulphate for ten minutes. Subsequently they were washed in distilled water for 30 minutes and finally fixed in a 5% solution of sodium thiosulphate. No additional staining was used. The sections were then mounted with glycerine jelly. The resulting lead lines are sharp and therefore conducive to quantitative measurements. Because of the relatively thin sections cut, histologic details can be observed.

16 citations

Journal ArticleDOI
TL;DR: The results provide a rationale for an inhibitory role of DM supplement and regular exercise in the attenuation of lead-induced neurotoxicity and significantly increased BDNF and TAC levels, as compared to the lead acetate group.
Abstract: Lead is a highly neurotoxic agent that particularly affects the developing central nervous system. In the current study we investigated the neuroprotective effects of exercise training and/or diferuloyl methane (DM) supplement, which is known as curcumin, on lead acetate-induced neurotoxicity in the rat hippocampus. Sixty rats were randomly divided into six groups: 1) lead acetate, 2) DM supplement, 3) endurance training, 4) training+ DM supplement, 5) sham and 6) base. The rats in the training groups performed treadmill running consisting of 15 to 22 m · min -1 for 25 to 64 min, 5 times a week for 8 weeks. All groups except sham received lead acetate (20 mg · kg -1 ), whereas the sham group received DM solvent. In addition, the DM and training+DM groups received DM solution (30 mg · kg -1 ) intraperitoneally. Chronic administration of lead acetate resulted in a significant increase in the malondialdehyde (MDA) in plasma, but not in the hippocampus. In addition, it led to significantly decreased brain-derived neurotrophic factor (BDNF) in the hippocampus and total antioxidant capacity (TAC) levels, as compared to the sham group. Treadmill running, DM supplementation, or both resulted in a significant decrease in MDA levels and significantly increased BDNF and TAC levels, as compared to the lead acetate group. These results provide a rationale for an inhibitory role of DM supplement and regular exercise in the attenuation of lead-induced neurotoxicity.

16 citations

Journal ArticleDOI
TL;DR: In this paper, the effect of lead acetate on nephrotoxicity and its correlation with the nitric oxide (NO) system by determining the NAG release in perfused rat kidney was investigated.

16 citations

Journal Article
TL;DR: Tetrabutyl lead was present in tissues in the highest quantities and lead acetate was the most poorly absorbed with the exception of lead oxide which demonstrated no absorption.
Abstract: Diffusion tubes were used to measure the degree of in vitro penetration of tetrabutyl lead, lead naphthanate, lead nuolate, lead acetate and lead oxide in excised guinea pig skin and human skin from autopsy. Tetrabutyl lead demonstrated the greatest penetration in skin from both guinea pig and man. Lead nuolate, lead naphthanate and lead acetate followed in descending order in the human tissue. A similar pattern occurred with guinea pig skin in most cases. There were no measurable amounts of lead oxide absorbed in either species. In vivo absorption was measured by applying 300 mg/kg tetrabutyl lead, lead nuolate, lead naphthanate or lead oxide to the shaved backs of guinea pigs for 7 d under occluded wrappings. Tetrabutyl lead was present in tissues in the highest quantities. Lead nuolate was present in greater amounts than lead naphthanate in the liver and kidneys. Lead acetate was the most poorly absorbed with the exception of lead oxide which demonstrated no absorption.

16 citations

10 Aug 2018
TL;DR: The present study suggests that 100 ppm oral doses of lead acetate II might have strong destructive effects on femur histology and osteocalcin expression.
Abstract: Lead is one of the harmful heavy metals that may be produced from human activities, and have deleterious effects on many tissues such as bone. In this research, the effects of oral administration of lead acetate II on histology of rat femur, and expression level of osteocaclcin gene were investigated. Twenty male Wistar rats were randomly divided into 2 groups. The rats in tested group were fed with 100 ppm of lead acetate II during 2 months. The femur samples were removed, fixed and then stained by alizarin red S for mineralization ratio assessment, and Hematoxylin-Eosin for histological studies. Also, real-time PCR was performed to determine the expression level of osteocalcin gene. The dose of 100 ppm of lead acetate II reduced mineralization and bone density, and decreased the relative density of osteoblasts. Also, the diameter of the bone marrow increased while the expression of osteocalcin gene decreased in tested group in comparison with the control group (P < 0.05). The present study suggests that 100 ppm oral doses of lead acetate II might have strong destructive effects on femur histology and osteocalcin expression.

16 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202352
2022110
202182
202087
201983
201887