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Leuconostoc mesenteroides

About: Leuconostoc mesenteroides is a research topic. Over the lifetime, 2389 publications have been published within this topic receiving 58513 citations.


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Journal ArticleDOI
TL;DR: Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera lactobacillus, Pediococcus, Leuconostoc, andWeissella in human feces.
Abstract: Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella in human feces. Analysis of fecal samples of four subjects revealed individual profiles of DNA fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria such as Lactobacillus sakei, Lactobacillus curvatus, Leuconostoc mesenteroides, and Pediococcus pentosaceus. Comparison of PCR-DGGE results with those of bacteriological culture showed that the food-associated species could not be cultured from the fecal samples by plating on Rogosa agar. On the other hand, all of the LAB species cultured from feces were detected in the DGGE profile. We also detected changes in the types of LAB present in human feces during consumption of a milk product containing the probiotic strain Lactobacillus rhamnosus DR20. The analysis of fecal samples from two subjects taken before, during, and after administration of the probiotic revealed that L. rhamnosus was detectable by PCR-DGGE during the test period in the feces of both subjects, whereas it was detectable by culture in only one of the subjects.

791 citations

Journal ArticleDOI
TL;DR: The production of homopolysaccharide producing thermophilic and mesophilic lactic acid bacteria, which are important in the dairy industry, and the produced polymers play a key role in the rheological behaviour and the texture of fermented milks are outlined.
Abstract: The production of homopolysaccharides (dextrans, mutans) and heteropolysaccharides by lactic acid bacteria, their chemical composition, their structure and their synthesis are outlined. Mutans streptococci, which include Streptococcus mutans and S. sobrinus produce soluble and insoluble α-glucans. The latter may contain as much as 90%α-1–3 linkages and possess a marked ability to promote adherence to the smooth tooth surface causing dental plaque. Dextrans produced by Leuconostoc mesenteroides are high molecular weight α-glucans having 1–6, 1–4 and 1–3 linkages, varying from slightly to highly branched; 1–6 linkages are predominant. Emphasis is put on exopolysaccharide producing thermophilic and mesophilic lactic acid bacteria, which are important in the dairy industry. The produced polymers play a key role in the rheological behaviour and the texture of fermented milks. One of the main problems in this field is the transitory nature of the thickening trait. This instability is not yet completely understood. Controversial results exist on the sugar composition of the slime produced, but galactose and glucose have always been identified with galactose predominating in most cases.

499 citations

Journal ArticleDOI
TL;DR: In this study, metagenomic approaches were used to monitor changes in bacterial populations, metabolic potential, and overall genetic features of the microbial community during the 29-day fermentation process of Kimchi, and provide insights into the kimchi microbial community.
Abstract: Kimchi, a traditional food in the Korean culture, is made from vegetables by fermentation. In this study, metagenomic approaches were used to monitor changes in bacterial populations, metabolic potential, and overall genetic features of the microbial community during the 29-day fermentation process. Metagenomic DNA was extracted from kimchi samples obtained periodically and was sequenced using a 454 GS FLX Titanium system, which yielded a total of 701,556 reads, with an average read length of 438 bp. Phylogenetic analysis based on 16S rRNA genes from the metagenome indicated that the kimchi microbiome was dominated by members of three genera: Leuconostoc, Lactobacillus, and Weissella. Assignment of metagenomic sequences to SEED categories of the Metagenome Rapid Annotation using Subsystem Technology (MG-RAST) server revealed a genetic profile characteristic of heterotrophic lactic acid fermentation of carbohydrates, which was supported by the detection of mannitol, lactate, acetate, and ethanol as fermentation products. When the metagenomic reads were mapped onto the database of completed genomes, the Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 and Lactobacillus sakei subsp. sakei 23K genomes were highly represented. These same two genera were confirmed to be important in kimchi fermentation when the majority of kimchi metagenomic sequences showed very high identity to Leuconostoc mesenteroides and Lactobacillus genes. Besides microbial genome sequences, a surprisingly large number of phage DNA sequences were identified from the cellular fractions, possibly indicating that a high proportion of cells were infected by bacteriophages during fermentation. Overall, these results provide insights into the kimchi microbial community and also shed light on fermentation processes carried out broadly by complex microbial communities.

396 citations

Journal ArticleDOI
TL;DR: This review covers the production, properties and applications of the biopolysaccharide dextran; this biopolymer can be produced via fermentation either with Leuconostoc mesenteroides strains and other lactic acid bacteria or with certain Gluconobacter oxydans strains.
Abstract: This review covers the production, properties and applications of the biopolysaccharide dextran; this biopolymer can be produced via fermentation either with Leuconostoc mesenteroides strains and other lactic acid bacteria or with certain Gluconobacter oxydans strains. The former strains convert sucrose into dextran with the dextransucrase enzyme whereas the latter convert maltodextrins into dextran with the dextran dextrinase enzyme. Emphasis is mainly focused on Leuconostoc strains as producer organisms of dextransucrase and dextran types. In addition to industrial fermentation processes producing the enzymes and/or the dextrans, biocatalysis principles are also being developed, whereby enzyme preparations convert sucrose or maltodextrins, respectively, into (oligo)dextrans. The chemical and physical properties of different dextrans are discussed in detail, together with the characteristics and molecular mode of action of dextransucrase. Subsequently, useful applications of dextran and some problems associated with undesirable formation of dextran are outlined. Copyright © 2005 Society of Chemical Industry

389 citations

Journal ArticleDOI
TL;DR: There was complete correlation over a serum glucose range of 4 to 12 mmol/liter (72 to 217 mg/dl) and use of NAD+ with I from L. mesenteroides is advantageous because of the decreased cost and greater stability of the coenzyme.
Abstract: The hexokinase (EC 2.7.1.1)/glucose-6-phosphate dehydrogenase (I) (EC 1.1.1.49) method of glucose analysis is highly accurate, precise, and sensitive when I from Leuconostoc mesenteroides and NAD+ are used. Coefficients of variation ranged from 0.68 to 8.9% for glucose standards between 0.56 and 27.7 mmol/liter. Recovery was 103 ± 10% for glucose standards added to sera of known glucose content. Although no substances investigated interfere with the test system when present at their normal or likely concentrations in serum, final reaction mixtures containing mannose (>0.28 mmol/liter), fructose (3.7 mmol/liter), or 2-deoxy-D-glucose (4.01 mmol/liter) inhibit the analysis. With all constituents lyophilized, the reagent mixture is stable for at least a year at 4 °C and for five days at 40 °C. Within-day reproducibility averaged 0.83%; day-to-day precision was 1.3%. We compared results of hexokinase/glucose procedures by using I from yeast (with NADP+) and from L. mesenteroides (with either NAD+ or NADP+ as coenzyme); there was complete correlation over a serum glucose range of 4 to 12 mmol/liter (72 to 217 mg/dl). Use of NAD+ with I from L. mesenteroides is advantageous because of the decreased cost and greater stability of the coenzyme.

377 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202356
2022127
202164
202080
201984
201880