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Lipid bilayer

About: Lipid bilayer is a research topic. Over the lifetime, 24734 publications have been published within this topic receiving 1075030 citations.


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Journal ArticleDOI
TL;DR: The possible functional roles of lipids are reviewed in terms of previous models such as the fluid mosaic model of Singer and Nicolson or the earlier unit membrane model so that the requirement for an alternative approach becomes apparent.

1,751 citations

Journal ArticleDOI
TL;DR: A coherent thermodynamic formalism for determining and describing the energetics of peptide-bilayer interactions and a review of the properties of the environment of membrane proteins--the bilayer milieu are reviewed.
Abstract: ▪ Abstract Stably folded membrane proteins reside in a free energy minimum determined by the interactions of the peptide chains with each other, the lipid bilayer hydrocarbon core, the bilayer interface, and with water. The prediction of three-dimensional structure from sequence requires a detailed understanding of these interactions. Progress toward this objective is summarized in this review by means of a thermodynamic framework for describing membrane protein folding and stability. The framework includes a coherent thermodynamic formalism for determining and describing the energetics of peptide-bilayer interactions and a review of the properties of the environment of membrane proteins—the bilayer milieu. Using a four-step thermodynamic cycle as a guide, advances in three main aspects of membrane protein folding energetics are discussed: protein binding and folding in bilayer interfaces, transmembrane helix insertion, and helix-helix interactions. The concepts of membrane protein stability that emerge p...

1,703 citations

Journal ArticleDOI
01 Jan 1980
TL;DR: This research attacked the mode of action of phosphatidylcholine-like deposits in response to the presence of ribonucleic acid by exploiting its role as a “spatially aggregating substance” in the response to EMT.
Abstract: IAbbreviations used in this article are as follows: AraC= l -,B-d arabinofuranosyl cytosine, Chol=cholesterol, DNA=deoxyribonucleic acid, DMPA=dimyristoyl phos­ phatidic acid, DMPC = dimyristoyl phosphatidylcholine, DMPE = dimyristoyl phos­ phatidylethanolamine, DOPC = dioleoyl phosphatidylcholine, DOPE = dioleoyl phos­ phatidylethanolamine, DPPA=dipaJmitoyl phosphatidic acid, DPPC=dipaJmitoyl phos­ phatidylcholine, DPPG = dipaJmitoyl phosphatidylglycerol, DPPS;= dipalmitoyl phos­ phatidylserine, DSPC = distearoyl phosphatidylcholine, EPC = egg phosphatidylcholine, EDTA=ethylene diamine tetracetic acid, HDL=high density lipoprotein, HPLC=high performance liquid chromatography, LUV = large unilamellar vesicle, MLV = multilamellar vesicle, NT A = nitrilotriacetic acid, NMR = nuclear magnetic resonance, PA phosphatidic acid, PC = phosphatidylcholine, PE = phosphatidylethanolamine, PO = phosphatidylglycerol, PS = phosphatidylserine, REV = reverse-phase evaporation vesicle, RNA = ribonucleic acid, SUV=small unilameUar vesicle, Tc=transition temperature. 2Present address: Department of Physiology, Tufts University School of Medicine, Boston, Massachusetts 02111.

1,666 citations

Journal ArticleDOI
TL;DR: This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what isknown of domain formation in cell membranes.
Abstract: Views of how cell membranes are organized are presently changing. The lipid bilayer that constitutes these membranes is no longer understood to be a homogeneous fluid. Instead, lipid assemblies, termed rafts, have been introduced to provide fluid platforms that segregate membrane components and dynamically compartmentalize membranes. These assemblies are thought to be composed mainly of sphingolipids and cholesterol in the outer leaflet, somehow connected to domains of unknown composition in the inner leaflet. Specific classes of proteins are associated with the rafts. This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what is known of domain formation in cell membranes.

1,615 citations

Journal ArticleDOI
23 Oct 2003-Nature
TL;DR: It is shown that high-resolution fluorescence imaging using two dyes preferentially labelling different fluid phases directly provides a correlation between domain composition and local membrane curvature, which is able to provide experimental estimates of boundary tension between fluid bilayer domains.
Abstract: Lipid bilayer membranes--ubiquitous in biological systems and closely associated with cell function--exhibit rich shape-transition behaviour, including bud formation and vesicle fission. Membranes formed from multiple lipid components can laterally separate into coexisting liquid phases, or domains, with distinct compositions. This process, which may resemble raft formation in cell membranes, has been directly observed in giant unilamellar vesicles. Detailed theoretical frameworks link the elasticity of domains and their boundary properties to the shape adopted by membranes and the formation of particular domain patterns, but it has been difficult to experimentally probe and validate these theories. Here we show that high-resolution fluorescence imaging using two dyes preferentially labelling different fluid phases directly provides a correlation between domain composition and local membrane curvature. Using freely suspended membranes of giant unilamellar vesicles, we are able to optically resolve curvature and line tension interactions of circular, stripe and ring domains. We observe long-range domain ordering in the form of locally parallel stripes and hexagonal arrays of circular domains, curvature-dependent domain sorting, and membrane fission into separate vesicles at domain boundaries. By analysing our observations using available membrane theory, we are able to provide experimental estimates of boundary tension between fluid bilayer domains.

1,535 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023635
20221,043
2021830
2020844
2019777
2018833