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Low protein

About: Low protein is a research topic. Over the lifetime, 8139 publications have been published within this topic receiving 213225 citations.


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Journal ArticleDOI
TL;DR: New solubilizing molecules, non-detergent sulphobetaines, are tested to improve the renaturation of two very different enzymes, hen egg white lysozyme and bacterial beta-D-galactosidase.

80 citations

Journal ArticleDOI
TL;DR: Two methods for estimating the flow of microbial protein synthesized in the rumen to the duodenum were compared: one uses microbial nucleic acids entering the duODenum, and the other uses allantoin excreted in the urine.
Abstract: Two methods for estimating the flow of microbial protein synthesized in the rumen to the duodenum were compared: one uses microbial nucleic acids entering the duodenum, and the other uses allantoin excreted in the urine. Ten ewes were fitted with rumen and duodenum cannulae, as well as Foley catheters for collection of urine. The experiment was carried out using two series of treatments with two replications each. The ewes were randomly divided into five groups, which were assigned to one of five diets. (In the second series sheep were excluded from diets received in the first series.) The diets, differing in protein and energy content, were as follows: (1) low protein, low energy (LPLE); high protein, low energy (HPLE); (3) maintenance for protein and energy (MPME); (4) low protein, high energy (LPHE); and (5) high protein, high energy (HPHE). The rates of rumen microbial protein synthesis were 3.34, 7.00, 9.44, 4.47 and 13.44 g microbial nitrogen (N) d−1 for diets 1–5, respectively. Results indicated a ...

80 citations

Journal ArticleDOI
TL;DR: In order to elucidate the renal mechanism for the regulation of urea excretion the urea clearance and the GFR were studied in sheep during normal and low protein intake in a range of urine flows.
Abstract: In order to elucidate the renal mechanism for the regulation of urea excretion the urea clearance and the GFR were studied in sheep during normal and low protein intake in a range of urine flows fr...

80 citations

Journal ArticleDOI
TL;DR: The effects of pumpkin seed (Cucurbita pepo) protein isolate on the plasma activity levels of catalase, superoxide dismutase, glutathione peroxidase, and total antioxidant capacity as well as glucose‐6‐phosphatase (G6Pase) in liver homogenates and lipid peroxidation levels are investigated.
Abstract: The effects of pumpkin seed (Cucurbita pepo) protein isolate on the plasma activity levels of catalase (CA), superoxide dismutase (SOD), glutathione peroxidase (GSHpx) and total antioxidant capacity (TAC) as well as glucose-6-phosphatase (G6Pase) in liver homogenates and lipid peroxidation (LPO-malondialdehyde-MDA) levels in liver homogenates and liver microsomal fractions against carbon tetrachloride (CCl4)-induced acute liver injury in low-protein fed Sprague-Dawley rats (Rattus norvegicus) were investigated. A group of male Sprague-Dawley rats maintained on a low-protein diet for 5 days were divided into three subgroups. Two subgroups were injected with carbon tetrachloride and the other group with an equivalent amount of olive oil. Two hours after CCl4 intoxication one of the two subgroups was administered with pumpkin seed protein isolate and thereafter switched onto a 20% pumpkin seed protein isolate diet. The other two groups of rats were maintained on the low-protein diet for the duration of the investigation. Groups of rats from the different subgroups were killed at 24, 48 and 72 h after their respective treatments. After 5 days on the low-protein diet the activity levels of all the enzymes as well as antioxidant levels were significantly lower than their counterparts on a normal balanced diet. However, a low-protein diet resulted in significantly increased levels of lipid peroxidation. The CCl4 intoxicated rats responded in a similar way, regarding all the variables investigated, to their counterparts on a low-protein diet. The administration of pumpkin seed protein isolate after CCl4 intoxication resulted in significantly increased levels of all the variables investigated, with the exception of the lipid peroxidation levels which were significantly decreased. From the results of the present study it is concluded that pumpkin seed protein isolate administration was effective in alleviating the detrimental effects associated with protein malnutrition and CCl4 intoxication. It is therefore apparent that pumpkin seed protein isolate has components that have antiperoxidative properties. Copyright © 2006 John Wiley & Sons, Ltd.

80 citations

Journal ArticleDOI
TL;DR: Plant‐specific quantitative methods such as metabolic labeling methods that can take full advantage of plant metabolism and culture practices are described, and other potential advantages and challenges that may arise from the unique properties of plants are discussed.
Abstract: Quantitation is an inherent requirement in comparative proteomics and there is no exception to this for plant proteomics. Quantitative proteomics has high demands on the experimental workflow, requiring a thorough design and often a complex multi-step structure. It has to include sufficient numbers of biological and technical replicates and methods that are able to facilitate a quantitative signal read-out. Quantitative plant proteomics in particular poses many additional challenges but because of the nature of plants it also offers some potential advantages. In general, analysis of plants has been less prominent in proteomics. Low protein concentration, difficulties in protein extraction, genome multiploidy, high Rubisco abundance in green tissue, and an absence of well-annotated and completed genome sequences are some of the main challenges in plant proteomics. However, the latter is now changing with several genomes emerging for model plants and crops such as potato, tomato, soybean, rice, maize and barley. This review discusses the current status in quantitative plant proteomics (MS-based and non-MS-based) and its challenges and potentials. Both relative and absolute quantitation methods in plant proteomics from DIGE to MS-based analysis after isotope labeling and label-free quantitation are described and illustrated by published studies. In particular, we describe plant-specific quantitative methods such as metabolic labeling methods that can take full advantage of plant metabolism and culture practices, and discuss other potential advantages and challenges that may arise from the unique properties of plants.

80 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20227
2021298
2020300
2019278
2018308
2017306