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Low protein
About: Low protein is a research topic. Over the lifetime, 8139 publications have been published within this topic receiving 213225 citations.
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TL;DR: The data suggest that substituting high-protein with low-protein feed in the nursery phase in aBiofloc dominated system operated with minimal discharge may provide an alternative to improve shrimp biofloc technology, through improved water quality, cheaper (lower protein) feed and reduced environmental impact.
69 citations
TL;DR: The new procedure eliminates the interference caused by Triton X-100, phospholipids, or dithiothreitol providing yields higher than 95% and seems to be especially suitable for protein determination on membrane preparations in samples with small volumes and/or very low protein concentrations.
69 citations
TL;DR: The data demonstrate that both restriction and excess dietary protein during pregnancy alters the offspring’s epigenetic marks and influences gene expression.
Abstract: Recent evidence indicates that maternal nutrition during pregnancy influences gene expression in offspring through epigenetic alterations. In the present study we evaluated the effect of protein excess and deficiency during porcine pregnancy on offspring hepatic and skeletal muscular expression patterns of key genes of methionine metabolism (DNMT1, DNMT3a, DNMT3b, BHMT, MAT2B and AHCYL1), condensin I subunit genes (NCAPD2, NCAPG and NCAPH), important for chromosome condensation and segregation, global DNA methylation and gene-specific DNA methylation. German Landrace sows were randomly assigned to control (CO), high protein (HP) and low protein (LP) diet groups. Tissue samples of offspring were collected from fetal (dpc95), newborn (dpn1), weanling (dpn28) and finisher pigs (dpn188). Gene expression of DNMT1, DNMT3a and DNMT3b was influenced by both HP and LP diets, indicating an involvement of DNA methylation in fetal programming by maternal protein supply. Moreover, hepatic global methylation was significantly affected by protein restriction at dpc95 (p = 0.004) and by protein excess at dpn188 (p = 0.034). Gene expression in fetal liver was significantly different between CO and LP for NCAPD2 (p = 0.0005), NCAPG (p = 0.0009) and NCAPH (p < 0.0001). In skeletal muscle, LP fetuses had significantly altered gene expression of NCAPD2 (p = 0.020) and NCAPH (p = 0.001), compared with CO. Furthermore, NCAPG was differentially methylated among LP, HP and CO; indeed, a significant positive correlation was detected with transcript amount in fetal pigs (r = 0.47, p = 0.002). These data demonstrate that both restriction and excess dietary protein during pregnancy alters the offspring's epigenetic marks and influences gene expression.
69 citations
TL;DR: In this article, the protein content of human urine is only 1% that of male rat urine, while the analogous fraction of rat urine containing αG, contained 26% of total urinary protein, and at pH 5.0, the most cationic fraction of human urinary protein constituted only about 4% of the total protein.
69 citations
TL;DR: In this article, the authors conducted a microarray study to identify genes and pathways differentially modulated in peripheral blood leukocytes isolated from 12 immunoglobulin A nephropathy (IgAN) patients and 8 healthy controls.
69 citations