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Low protein

About: Low protein is a research topic. Over the lifetime, 8139 publications have been published within this topic receiving 213225 citations.


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Journal ArticleDOI
TL;DR: A comparison of SEC refolding with refolding by batch dilution is conducted, using lysozyme as a model protein, and it is found that, contrary to previous reports, size-exclusion refolding on batch columns leads to refolding yields slightly better than batch dilutionsRefolding yields at low protein concentrations but this advantage disappears at higher protein concentrations.

63 citations

Journal ArticleDOI
TL;DR: In this paper, an insight is given in the legislation policy to restrain environmental pollution by pig husbandry, focused on The Netherlands (Mineral Accounting System), and nutritional measures are presented to reduce environmental pollution, by lowering excretion of N and P, emphasizing (multi) phase feeding, the use of low protein, synthetic amino acids supplemented diets, phytase and its effect on phosphorus and calcium digestibility, its interaction with phytic acid and proteins.
Abstract: In the paper insight is given in the legislation policy to restrain environmental pollution by pig husbandry, focused on The Netherlands (Mineral Accounting System). Besides, nutritional measures are presented to reduce environmental pollution by lowering excretion of N and P, emphasizing (multi) phase feeding, the use of low protein, synthetic amino acids supplemented diets, phytase and its effect on phosphorus and calcium digestibility, its interaction with phytic acid and proteins, and the environmental impact of the use of phytase in pig diets. Also, nutritional means are indicated to reduce ammonia volatilization from pig operations. It is concluded that nutrition management can substantially contribute to reduction of N and P excretion by pigs, mainly by lowering dietary protein levels, (multi) phase feeding and the use of microbial phytase, and that the use of phytase on a large scale in The Netherlands has a tremendous environmental impact. In 20 years the excretion of P in growing-finishing pigs has more than halved. Ammonia emission from manure of pigs can be reduced substantially by lowering dietary protein content, but also by including additional non-starch polysaccharides in the diet. A very promising method to reduce ammonia emission is to manipulate dietary cation-anion difference, e.g. by adding acidifying salts to the diet, which will lower pH of urine substantially. Further research is desirable. This also applies to determining dietary factors influencing the odour release from manure. Finally, some speculation on the future of pig farming from an environmental viewpoint is presented.

63 citations

Journal ArticleDOI
TL;DR: Northern blots showed that refeeding after a prolonged starvation rapidly reverses the glucose/glucose-6-phosphate substrate cycle flux in the fish liver through decreased G6Pase expression and strong glucokinase (GK) induction, indicating that hepatic intermediary metabolism of fish fed diets with low protein/high carbohydrate diets is impelled towards utilization of dietary carbohydrates.
Abstract: To examine the relationship between structure and function of glucose-6-phosphatase (G6Pase) in fish, we undertook molecular cloning and modulation of G6Pase expression by starvation and refeeding on diets with different nutrient composition in the liver of the carnivorous fish, Sparus aurata. A cDNA encoding the full-length G6Pase catalytic subunit from the liver of S. aurata was isolated. This cDNA encodes a 350-amino acid protein, with low homology to the mammalian G6Pase, although it contains most of the key residues required for catalysis. Based on hydrophobicity and membrane structure prediction, we propose a model containing nine-transmembrane regions for S. aurata G6Pase. Northern blots showed that refeeding after a prolonged starvation rapidly reverses the glucose/glucose-6-phosphate substrate cycle flux in the fish liver through decreased G6Pase expression and strong glucokinase (GK) induction. The effect of refeeding different diets on G6Pase and GK expression, indicated that hepatic intermediary metabolism of fish fed diets with low protein/high carbohydrate diets is impelled towards utilization of dietary carbohydrates, by means of modulation of GK mRNA levels rather than G6Pase expression. These findings challenge the role attributed to dysregulation of G6Pase or GK expression in the low ability of carnivorous fish to metabolise glucose.

63 citations

Journal ArticleDOI
TL;DR: The present study underlines the importance of cultivation conditions and shows the practicability of microalgae biomass usage as mono-substrate for highly efficient continuous fermentation to methane without any pretreatment with almost maximum practically achievable energy conversion efficiency (biomass to methane).
Abstract: The fact that microalgae perform very efficiently photosynthetic conversion of sunlight into chemical energy has moved them into the focus of regenerative fuel research. Especially, biogas generation via anaerobic digestion is economically attractive due to the comparably simple apparative process technology and the theoretical possibility of converting the entire algal biomass to biogas/methane. In the last 60 years, intensive research on biogas production from microalgae biomass has revealed the microalgae as a rather challenging substrate for anaerobic digestion due to its high cell wall recalcitrance and unfavorable protein content, which requires additional pretreatment and co-fermentation strategies for sufficient fermentation. However, sustainable fuel generation requires the avoidance of cost/energy intensive biomass pretreatments to achieve positive net-energy process balance. Cultivation of microalgae in replete and limited nitrogen culture media conditions has led to the formation of protein-rich and low protein biomass, respectively, with the last being especially optimal for continuous fermentation. Anaerobic digestion of nitrogen limited biomass (low-N BM) was characterized by a stable process with low levels of inhibitory substances and resulted in extraordinary high biogas, and subsequently methane productivity [750 ± 15 and 462 ± 9 mLN g−1 volatile solids (VS) day−1, respectively], thus corresponding to biomass-to-methane energy conversion efficiency of up to 84%. The microbial community structure within this highly efficient digester revealed a clear predominance of the phyla Bacteroidetes and the family Methanosaetaceae among the Bacteria and Archaea, respectively. The fermentation of replete nitrogen biomass (replete-N BM), on the contrary, was demonstrated to be less productive (131 ± 33 mLN CH4 g−1VS day−1) and failed completely due to acidosis, caused through high ammonia/ammonium concentrations. The organization of the microbial community of the failed (replete-N) digester differed greatly compared to the stable low-N digester, presenting a clear shift to the phyla Firmicutes and Thermotogae, and the archaeal population shifted from acetoclastic to hydrogenotrophic methanogenesis. The present study underlines the importance of cultivation conditions and shows the practicability of microalgae biomass usage as mono-substrate for highly efficient continuous fermentation to methane without any pretreatment with almost maximum practically achievable energy conversion efficiency (biomass to methane).

63 citations

Journal ArticleDOI
TL;DR: A capillary zone electrophoresis method is described for the analysis of fish sarcoplasmic proteins at low pH; the CZE protein profiles that resulted were specific for each species and could be employed for differentiation and identification purposes.
Abstract: A capillary zone electrophoresis (CZE) method is described for the analysis of fish sarcoplasmic proteins at low pH. Aqueous extracts of eight flatfish species, containing the sarcoplasmic proteins, were subjected to the action of an electric field in a capillary electrophoresis apparatus ; the separated proteins were detected by means of an UV detector after less than 35 min. Acceptable resolution and reproducibility were obtained using low protein concentration (0.1 mg/mL) in the extracts analyzed. The CZE protein profiles that resulted were specific for each species and could be employed for differentiation and identification purposes.

63 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20227
2021298
2020300
2019278
2018308
2017306