Topic
Lysis
About: Lysis is a research topic. Over the lifetime, 6072 publications have been published within this topic receiving 216978 citations.
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TL;DR: Chinese hamster ovary cells in exponential growth were incubated with various concentrations of hematoporphyrin derivative, and lysis of the cell membrane may be largely responsible for cellular inactivation following HpD photoirradiation.
Abstract: Chinese hamster ovary cells in exponential growth were incubated with various concentrations of hematoporphyrin derivative (HpD). Cellular porphyrin content was determined after 2 h incubation at 37°C using [3H]-hematoporphyrin derivative. Photoactivation of cell-bound HpD by red light resulted in a family of survival curves with terminal slopes proportional to cellular HpD concentration. The degree of cellular lysis, assayed 1 h after illumination using a chromium-51 labeling technique, was also found to be related to cellular HpD concentration. The amount of 51Cr released increased with post-irradiation incubation to a level parallel to cell lethality as measured by colony formation. These data suggest that lysis of the cell membrane may be largely responsible for cellular inactivation following HpD photoirradiation.
82 citations
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TL;DR: Evidence is presented to show that the structural genes for enzyme production are located on the phage genome rather than on the bacterial genome, and one of the enzyme systems was unusual in that it was only produced following phage infection and lysis of mucoid host strains.
Abstract: SUMMARY: Several bacteriophages have been isolated which, in association with the host bacteria, produce enzymes that depolymerize the exopolysaccharide of Escherichia coli K12 and other slime polysaccharides of the same chemical type. Chemical analyses show the similarity of the polysaccharides produced by E. coli K12, by other E. coli strains and by Aerobacter cloacae NCTC 5920: all contain 28-33% fucose, 16-19% glucose, 25-28% galactose, 14-22% glucuronic acid. The action of the depolymerizing enzymes greatly decreases the viscosity of the polysaccharide solutions but does not liberate any fragments of low molecular weight. Partial purification of the enzymes was achieved by ammonium sulphate precipitation and chromatography on DEAE-cellulose. The enzymes are active against exopolysaccharides produced by bacteria in which the phages are unable to multiply. Evidence is presented to show that the structural genes for enzyme production are located on the phage genome rather than on the bacterial genome. One of the enzyme systems was unusual in that it was only produced following phage infection and lysis of mucoid host strains. Its production was induced by the polysaccharide substrate.
82 citations
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TL;DR: A simple inexpective method to study the kinetics of complement-mediated immune lysis of liposomes containing sheep red blood cell lipid antigens based on the fact that trapping the fluorescent molecule 1-aminonaphthalene-3,6,8-trisulfonate and the dynamic quencher within the liposome inner volume results in an extinguished fluorescence signal.
82 citations
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TL;DR: The kinetics of uridine incorporation into RNA of uninfected and T4-infected bacteria and study of the net synthesis of bacterial RNA at different uridine concentrations in the medium gave the following results: theNet synthesis rate ofacterial RNA (in uninfecting cells and the RNA chain growth in infected cells are independent of the uridine concentration in themedium.
82 citations
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TL;DR: Sensitive bacteria infected with mutant strains of phage T4 that lack active lysozyme do not lyse at the usual time, but oxygen uptake in these bacteria cease at the time lysis would normally occur, suggesting superinfection that causes lysis inhibition prolongs the time during which oxygen uptake occurs.
82 citations