Topic
Lysis
About: Lysis is a research topic. Over the lifetime, 6072 publications have been published within this topic receiving 216978 citations.
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Patent•
08 Jul 1994
TL;DR: In this article, a method for purifying from cells a target compound such as nucleic acid is provided, which comprises the following steps: 1) lysing a cell suspension to form a cell lysate containing the target compound, 2) applying the cell lysis to a filter to remove unwanted cells and cell debris, 3) contacting the filtered lysatter with a solid phase matrix under conditions to bind the nucleic acids to the matrix, 4) separating the resultant filtered lysisate from the matrix; and 5) eluting the target compounds from the matrices
Abstract: A method is provided for purifying from cells a target compound such as nucleic acid. The method comprises the following steps: 1) lysing a cell suspension to form a cell lysate containing the target compound; 2) applying the cell lysate to a filter to remove unwanted cells and cell debris; 3) contacting the filtered lysate with a solid phase matrix under conditions to bind the nucleic acid to the matrix; 4) separating the resultant filtered lysate from the matrix; and 5) eluting the target compound from the matrix. Apparatus is also provided. Complex purification procedures such as centrifugation are avoided.
69 citations
TL;DR: The effect of human activated protein C (APC) on tissue plasminogen activator (tPA)-induced fibrinolysis was studied in cell free plasma and in a system of purified components and it was shown that the component with the required activity has properties of the procoagulant surface and can be replaced with vesicles of phosphatidylcholine/phosph atidylserine (PCPS).
69 citations
TL;DR: Electron microscopic analysis showed that the purified HBcAg assembled into particles that closely resemble the viral nucleocapsid form and reacted with anti-HBcAg monoclonal antibody in enzyme-linked immunosorbent assay (ELISA) showing that it is functionally active.
69 citations
Patent•
03 Feb 1995
TL;DR: A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy using an anion exchanger is described in this paper.
Abstract: A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein nucleic acids and/or oligonucleotides are isolated or purified from an essentially biological source, characterized in that essentially biological sources are lysed, the fractions obtained are optionally freed or depleted from the remainder of biological sources by per se known mechanical methods, such as centrifugation, filtration; the fractions thus treated are subsequently treated with affinity chromatographic material or with inorganic chromatographic material for the removal of endotoxins; followed by isolation of nucleic acids and/or oligonucleotides on an anion exchanger which is designed such that DNA begins to desorb from the anion exchanger only at an ionic strength corresponding to a sodium chloride solution of a concentration higher by at least 100 mM than one corresponding to the ionic strength at which RNA begins to desorb from the anion exchanger material.
69 citations