scispace - formally typeset
Search or ask a question
Topic

Lysis

About: Lysis is a research topic. Over the lifetime, 6072 publications have been published within this topic receiving 216978 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: Nine human cell types displayed a marked resistance to lysis by hydrogen peroxide, with both catalase and the GSH redox cycle inhibited, that is, resistance independent of detectable degradation of H2O2.
Abstract: Nine human cell types, six of them malignant, displayed a marked resistance to lysis by hydrogen peroxide (LD50, 2-20 mM). Of the reactive oxygen intermediates generated extracellularly, only H2O2 lysed all the cell types. OH was lytic to one of four, OI- to one of one, and O-2 to none of four cell types tested. Resistance to oxidative lysis did not correlate with specific activity of catalase, glutathione (GSH) peroxidase, other peroxidases, or glutathione disulfide reductase, or with specific content of GSH. Resistance to H2O2 seemed to occur via mechanisms distinct from those responsible for cellular consumption of H2O2. Consumption was inhibitable by azide and was probably due to catalase in each cell type. In contrast, resistance to oxidative lysis occurred via distinct routes in different cells. One cell type used the GSH redox cycle as the primary defense against H2O2, like murine tumors previously studied. Other cells seemed to utilize catalase as the major defense against H2O2. Nonetheless, with both catalase and the GSH redox cycle inhibited, all the human cells tested exhibited an inherent resistance to oxidative lysis, that is, resistance independent of detectable degradation of H2O2.

64 citations

Journal ArticleDOI
TL;DR: Both the amount and the chemical composition of EPS strongly depended on the extraction method used, and the major constituents were proteins and carbohydrates, showing the major presence of carboxylic, hydroxylic and amino groups.

64 citations

Journal ArticleDOI
TL;DR: This method is suitable for assessing gene and protein expression from a single brain sample, allowing for a more comprehensive evaluation of neural phenomena while minimizing the number of subjects.

64 citations

Journal ArticleDOI
TL;DR: The results suggest that membrane immunoglobulins of T and B lymphocytes are structurally distinct and that T cell Ig may be more hydrophobic than its B cell counterpart.

64 citations


Network Information
Related Topics (5)
Cell culture
133.3K papers, 5.3M citations
86% related
Antigen
170.2K papers, 6.9M citations
86% related
DNA
107.1K papers, 4.7M citations
86% related
Immune system
182.8K papers, 7.9M citations
84% related
Gene
211.7K papers, 10.3M citations
83% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023389
2022607
2021123
2020142
2019139
2018161