Topic
Lysis
About: Lysis is a research topic. Over the lifetime, 6072 publications have been published within this topic receiving 216978 citations.
Papers published on a yearly basis
Papers
More filters
••
TL;DR: A number of hydroxylamine-induced temperature-sensitive mutants of φX174, which do not complement each other functionally and are probably all altered in coat protein structure, do not form progeny at 41°C, and lysis of cells infected with these mutants however is not affected.
57 citations
•
TL;DR: The effector-dependent action on the target cell is completed much earlier than evidenced by chromium-51 release, and subsequent, effectorindependent steps must occur before completion of target cell lysis.
Abstract: Heparin was previously shown to inhibit the lysis of target cells by sensitized thymus-derived mouse lymphocytes in vitro. However, heparin failed to inhibit target cell lysis when added to the cultures after only 10% specific release of chromium-51 had occurred. For further exploration of this phenomenon, effector cells have been specifically inactivated at various times during their action on target cells. This was accomplished with alloantiserum made in target strain mice (DBA/2) against effector strain cells (C57BL/6). Treatment of effector cells with this antiserum and complement rendered them unable to initiate new cytolytic events on target cells (and incidentally did not release soluble toxins). However, when untreated cultures were incubated for about 60 min, after which the effector cells had caused 10% specific release of chromium-51 from the target population, treatment of the cultures with anti-effector serum and complement did not interfere with the subsequent lysis of more than half of the target cell population. Hence, the effector-dependent action on the target cell is completed much earlier than evidenced by chromium-51 release, and subsequent, effectorindependent steps must occur before completion of target cell lysis. Data suggest that divalent cations are required for the effector-dependent phase, but not for the effector-independent phase.
57 citations
••
TL;DR: A plasmid encoded novel bacteriocin BMP11 produced by Lactobacillus crustorum MN047 was innovatively identified and found to have rich α-helix conformation after prediction and had promising potential as antimicrobial to control foodborne pathogens in dairy products.
57 citations
••
TL;DR: A novel, continuous-flow, radially focused ultrasonic disruptor capable of lysing Bacillus spores in the absence of added chemical denaturants, enzymes, or microparticles is reported on.
Abstract: We report on the development of a novel, continuous-flow, radially focused ultrasonic disruptor capable of lysing Bacillus spores in the absence of added chemical denaturants, enzymes, or microparticles. Greater than 99% disruption was achieved for Bacillus globigii spores and Escherichia coli and Bacillus subtilis vegetative cells with sample residence times of 62, 12, and 12 s, respectively. Microscopic and SEM images indicated that at equivalent power levels, the incidence of cell death or loss of viability typically exceeded the efficiency of (visible) cell lysis. However, semiquantitative PCR showed up to a 1000-fold increase in intracellular DNA availability from ultrasonically disrupted spores, and liberated DNA was intact and available for subsequent detection.
57 citations
••
TL;DR: In Qbeta, the A2 protein, in addition to its functions as maturation protein, appears to trigger cell lysis, which contrasts with the situation in the distantly related group I RNA phages such as f2 and MS2 where a small lysis polypeptide is coded for by a region overlapping the end of the coat gene and the beginning of the replicase gene.
Abstract: Complete or partial cDNA sequences of the RNA bacteriophage Qbeta were cloned in plasmids under the control of the lambdaP(L) promoter to allow regulated expression in Escherichia coli harbouring the gene for the temperature-sensitive lambdaCI857 repressor. Induction of the complete Qbeta sequence leads to a 100-fold increase in phage production, accompanied by cell lysis. Induction of the 5'-terminal sequence containing the intact maturation protein (A2) cistron also causes cell lysis. Alterations of the A2 cistron, leading to proteins either devoid of approximately 20% of the C-terminal region or of six internal amino acids, abolish the lysis function. Expression of other cistrons in addition to the A2 cistron does not enhance host lysis. Thus, in Qbeta, the A2 protein, in addition to its functions as maturation protein, appears to trigger cell lysis. This contrasts with the situation in the distantly related group I RNA phages such as f2 and MS2 where a small lysis polypeptide is coded for by a region overlapping the end of the coat gene and the beginning of the replicase gene.
57 citations