Macrocybe crassa

About: Macrocybe crassa is a research topic. Over the lifetime, 7 publications have been published within this topic receiving 56 citations.

The pantropical genus macrocybe gen. nov.

Abstract: Analysis of morphological and ecological characters plus ribosomal DNA sequences support the removal of seven related tropical species from the genus Tricholoma. This group of apparently nonmycorrh...

Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa)

Abstract: . Inyod I, SassanarakitS, PayapanonA, Keawsompong S. 2017. Morphological characteristics and molecular identification of a wild Thai isolate of the tropical mushroom Hed Taen Rad (Macrocybe crassa). Biodiversitas 18: 221-228. Hed Taen Rad or Macrocybe crassa is a wild edible mushroom found in Thailand. It has a large fruiting body with a meaty texture and a delicious taste. Five strains of Hed Taen Rad; DOA, DOA-1, DOA-4, DOA-7 and DOA-10 were tested in different media, pH values and temperatures to measure mycelium growth kinetics and identify mycelia and basidiomata. Results indicated that mushroom mycelial growth rates were highest on MEA medium at pH values between 8 and 10, and temperatures between 20°C and 30°C. Identification of the isolates was based on the morphology characteristics of the basidiomata were closely similar to M. crassa (Berk.), and different from M. gigantea (Massee). For molecular analysis by sequencing of their internal transcribed spacer (ITS) regions was identified as a T. giganteum since limited ITS rDNA sequences from M. crassa exist in public databases. Therefore, the identification of five strains of Hed Taen Rad using molecular methods could be erroneous. Therefore, morphology characteristics study is suitable for further analysis method on genetic study of Hed Taen Rad.Keywords: Hed Taen Rad, Macrocybe crassa, molecular analysis, morphological characteristics

Pharmacognostic standardization of Macrocybe crassa: An imminent medicinal mushroom

Abstract: The aim of present study was to establish standardization parameters for pharmacognostic evaluation of Macrocybe crassa, a wild edible mushroom of West Bengal. For this purpose, microscopic features of powder such as physical characters, spore measurement, type of hyphae were examined and recorded. Physico-chemical parameters like organoleptic features, loss on drying (10.53%), total ash (17.04%), swelling capacity (66.66 ml/g), water holding capacity (174.69 g/g), water solubility index (406.65%) and fluorescent behaviour against 16 reagents were also determined. Alcoholic extract was prepared using methanol as solvent where extractive value was 27.52%. Preliminary mycochemical analysis indicated the presence of cardiac glycoside, carbohydrate, flavonoid, phenol, saponin and terpenoids while absence of alkaloid and steroid in methanol extract. While quantitatively major bioactive components were present in the following order of flavonoid> phenol> ascorbic acid> s-carotene> lycopene. In addition, HPTLC and HPLC profiles were recorded to determine phenolic fingerprint. HPTLC characterization showed presence of at least 5 components as detected by scanning the plate at 300 nm. HPLC chromatogram of the extract indicated existence of minimum 14 peaks which might be of phenolic compounds. Along with, DPPH radical scavenging activity (EC50 value 2.455 mg/ml) and total antioxidant capacity (7.4 ± 1.24 µg AAE/mg of extract) were determined to evaluate antioxidant activity. The above parameters, which are being reported for the first time, are significant towards establishing the pharmacognostic standards for future identification and authentication of genuine mushroom material.

Antioxidant and Antimicrobial Potentiality of Quantitatively Analysed Ethanol Extract from Macrocybe crassa

Abstract: Macrocybe crassa, a wild mushroom growing mainly in coastal areas of West Bengal, has been widely used as food for its flavour and taste. The present study was conducted to emphasize on antioxidant and antimicrobial activity as well as phenolic profile of crude ethanolic extract of this mushroom (EfraMac). Mycochemical analysis showed presence of bioactive components in the following order: phenol> flavonoid> ascorbic acid> β-carotene> lycopene. Further analysis with HPLC revealed that the dominant phenolic in EfraMac was pyrogallol, whereas vanillic acid, cinnamic acid and p-coumaric acid were present in less amounts. Antioxidant activity was determined by using superoxide radical scavenging, DPPH . radical inhibition, chelating ability of ferrous ion and total antioxidant activity assays. Promising results were obtained as evident by low EC50 values (ranging from 0.53 mg/ml to 1.66 mg/ml) and high ascorbic acid equivalent antioxidant capacity data. In addition, EfraMac exhibited antimicrobial effect against three Gram positive (Listeria monocytogenes, Staphylococcus aureus, Bacillus subtilis) and two Gram negative (Escherichia coli, Pseudomonas aeruginosa) bacteria, whereas Salmonella typhimurium was resistant to the fraction. Overall results indicated that the mushroom can serve as a good source of polyphenols in the human diet due to high concentrations of total phenolic compounds as well as their strong bioactivity. Moreover, the present study reports antioxidant and antimicrobial activity in relation with phenolic profile of Macrocybe crassa for the first time.