Topic
Macromolecular crowding
About: Macromolecular crowding is a research topic. Over the lifetime, 975 publications have been published within this topic receiving 48590 citations.
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TL;DR: Positive results of crowding include enhancing the collapse of polypeptide chains into functional proteins, the assembly of oligomeric structures and the efficiency of action of some molecular chaperones and metabolic pathways.
2,104 citations
TL;DR: Theoretical and experimental approaches to the characterization of crowding- and confinement-induced effects in systems approaching the complexity of living organisms are suggested.
Abstract: Expected and observed effects of volume exclusion on the free en- ergy of rigid and flexible macromolecules in crowded and confined systems, and consequent effects of crowding and confinement on macromolecular reaction rates and equilibria are summarized. Find- ings from relevant theoretical/simulation and experimental literature published from 2004 onward are reviewed. Additional complexity arising from the heterogeneity of local environments in biological media, and the presence of nonspecific interactions between macro- molecules over and above steric repulsion, are discussed. Theoretical and experimental approaches to the characterization of crowding- and confinement-induced effects in systems approaching the com- plexity of living organisms are suggested.
1,891 citations
TL;DR: This paper presents a meta-analyses of the macromolecular determinants of reaction rates and volume in a solution and describes the mechanisms leading to these rates and volumes.
Abstract: CONTENTS PERSPECTIVES AND OVERVIEW 28 Nomenclature 30 THEORETICAL CONCEPTS 31 The Effect of Macromolecular C owdin9 on Solution Equilibria 31 The Effect of Macromolecular C owdin# on Reaction Rates 36 The Effect of Macromolecular Crowding on Diffusive Transport of Solutes 37 The Effective Specific Volume of a Macromolecule--What Is It? 40
1,430 citations
TL;DR: To properly assess the physiological role of a particular reaction or set of reactions characterized in vitro, it is important to consider the possible influence of crowding and/or confinement upon the reaction in its physiological milieu.
1,362 citations
TL;DR: In this paper, the authors made an experimental study of the cytoplasm of Escherichia coli and used parameters from cells and cell extracts to calculate approximate activity coefficients for the thermodynamic activity of cellular components.
1,148 citations