scispace - formally typeset

Topic

Marinobacterium stanieri

About: Marinobacterium stanieri is a(n) research topic. Over the lifetime, 7 publication(s) have been published within this topic receiving 171 citation(s). The topic is also known as: P. stanieri.

Papers
More filters
Journal ArticleDOI
Abstract: The phylogenetic relationships of Oceanospirillum strains were analysed by using the nucleotide sequences of 16S rRNA and gyrB genes. Results from sequence analysis demonstrated that the Oceanospirillum core group consisted of four species, Oceanospirillum linum, Oceanospirillum maris, Oceanospirillum beijerinckii and Oceanospirillum multiglobuliferum, with enough distance to separate them as different species. However, four other Oceanospirillum species occupied taxonomic positions separate from the Oceanospirillum core group: Oceanospirillum jannaschii, Oceanospirillum japonicum and Oceanospirillum kriegii in the gamma-Proteobacteria and Oceanospirillum pusillum in the alpha-Proteobacteria. Oceanospirillum jannaschii clustered with Marinobacterium georgiense, Pseudomonas iners and Pseudomonas stanieri on the basis of phylogenetic analysis of 16S rRNA and gyrB genes. The other three species did not cluster with known genera. Also, the sequence similarity values of the gyrB genes between the three subspecies of Oceanospirillum maris and those between the two subspecies of Oceanospirillum beijerinckii were above 99%. The close relationships between the subspecies of Oceanospirillum maris and of Oceanospirillum beijerinckii were further supported by similar physiological properties and high DNA-DNA hybridization values, suggesting that these subspecies should not be regarded as valid. From these results, Oceanospirillum sensu stricto should be defined to consist of Oceanospirillum linum, Oceanospirillum maris, Oceanospirillum beijerinckii and Oceanospirillum multiglobuliferum. We propose to create the following new genera: Pseudospirillum gen. nov. for Oceanospirillum japonicum as Pseudospirillum japonicum comb. nov.; Oceanobacter gen. nov. for Oceanospirillum kriegii as Oceanobacter kriegii comb. nov.; and Terasakiella gen. nov. for Oceanospirillum pusillum as Terasakiella pusilla comb. nov. The transfer is proposed of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. nov. Furthermore, Pseudomonas iners should be reclassified as a strain of Marinobacterium georgiense. Finally, the subspecies of Oceanospirillum maris (O. maris subsp. maris, O. maris subsp. hiroshimense and O. maris subsp. williamsae) and Oceanospirillum beijerinckii (O. beijerinckii subsp. beijerinckii and O. beijerinckii subsp. pelagicum) should be combined as Oceanospirillum maris and Oceanospirillum beijerinckii, respectively.

84 citations

Journal ArticleDOI
TL;DR: A bacterial strain obtained from surface seawater collected near the coast of Deokjeok island (Yellow Sea), using a standard dilution-plating method, represents a novel species of the genus Marinobacterium, for which the name Marinobacteria litorale sp.
Abstract: A bacterial strain named IMCC1877T was obtained from surface seawater collected near the coast of Deokjeok island (Yellow Sea), using a standard dilution-plating method. The strain was Gram-negative, chemoheterotrophic and facultatively anaerobic, requiring NaCl, and cells were motile rods with a single polar flagellum. Colonies on marine agar were very small (average diameter 0.1 mm). Based on 16S rRNA gene sequences, the most closely related species to strain IMCC1877T was Marinobacterium stanieri (93.7 % sequence similarity to the type strain). Phylogenetic analyses based on 16S rRNA gene sequences showed that this marine isolate belonged to the order Oceanospirillales and formed an independent phyletic line within the clade forming the genus Marinobacterium. The DNA G+C content of the strain was 60.7 mol% and the predominant constituents of the cellular fatty acids were C18 : 1 ω7c (36.6 %), C16 : 1 ω7c and/or iso-C15 : 0 2-OH (26.7 %) and C16 : 0 (24.3 %). Based on the taxonomic data, only a distant relationship could be established between strain IMCC1877T and other Marinobacterium species; the strain therefore represents a novel species of the genus Marinobacterium, for which the name Marinobacterium litorale sp. nov. is proposed. The type strain is IMCC1877T (=KCTC 12756T=LMG 23872T).

32 citations

Journal ArticleDOI
TL;DR: It is proposed that strain AR11(T) (=KCTC 22254(T)=JCM 15134(T)) represents the type strain of a novel species, Marinobacterium maritimum sp.
Abstract: A Gram-negative, aerobic, rod-shaped, motile, marine bacterium, strain AR11T, was isolated from Arctic marine sediment. Strain AR11T grew with 0.5–7 % NaCl and at 7–37 °C and pH 5.5–9.0. It utilized propionate, 3-hydroxybenzoate, l-proline, acetate, d- and l-lactate, l-alanine, malate and phenylacetic acid. Alkaline phosphatase, esterase lipase (C8), leucine arylamidase and acid phosphatase activity tests were positive. Acid was produced from 5-ketogluconate and aesculin. Strain AR11T possessed C16 : 0 (22.0 %), summed feature 4 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH; 28.1 %) and summed feature 7 (one or more of C18 : 1 ω7c, ω9t and ω12t; 34.0 %) as the major cellular fatty acids. The major ubiquinone was Q-8. Comparative 16S rRNA gene sequence studies showed that strain AR11T belonged to the Gammaproteobacteria and was most closely related to Marinobacterium stanieri DSM 7027T, Marinobacterium halophilum mano11T and Marinobacterium georgiense KW-40T (97.8, 97.0 and 96.7 % similarity, respectively). The G+C content of the genomic DNA of strain AR11T was 57.9 mol%. DNA–DNA relatedness data indicated that strain AR11T represented a distinct species that was separated from M. stanieri DSM 7027T, M. halophilum KCTC 12240T and M. georgiense JCM 21667T. On the basis of evidence from this polyphasic study, it is proposed that strain AR11T (=KCTC 22254T=JCM 15134T) represents the type strain of a novel species, Marinobacterium maritimum sp. nov.

17 citations

Journal ArticleDOI
TL;DR: Two strains, CN44(T) and CN47(T), isolated from marine sediment of the East China Sea, were characterized by using a polyphasic approach and represent two novel species within the genus Marinobacterium, for which the names Marinobacteria nitratireducens sp.
Abstract: Two strains, CN44(T) and CN47(T), isolated from marine sediment of the East China Sea, were characterized by using a polyphasic approach. The isolates were Gram-negative, strictly aerobic, non-spore-forming rods. The chemotaxonomic characteristics of these isolates included the presence of C(18 : 1)omega7c, C(16 : 0), iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c and C(10 : 0) 3-OH as the major cellular fatty acids and Q-8 as the predominant ubiquinone. The DNA G+C contents of strains CN44(T) and CN47(T) were 62.5 and 56.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CN44(T) was related to members of the genus Marinobacterium. The most closely related described organism was the type strain of Marinobacterium rhizophilum (95.3 % sequence similarity). Strain CN47(T) showed the highest sequence similarity to the type strain of Marinobacterium stanieri (97.8 %) and <97 % similarity to other type strains of described Marinobacterium species. The level of DNA-DNA relatedness between strain CN47(T) and M. stanieri DSM 7027(T) was 46 %. On the basis of phenotypic and genotypic properties, strains CN44(T) and CN47(T) represent two novel species within the genus Marinobacterium, for which the names Marinobacterium nitratireducens sp. nov. (type strain, CN44(T) =CGMCC 1.7286(T) =JCM 15523(T)) and Marinobacterium sediminicola sp. nov. (type strain, CN47(T) =CGMCC 1.7287(T) =JCM 15524(T)) are proposed.

16 citations

Journal ArticleDOI
TL;DR: DNA-DNA hybridizations between strain R-40509(T) and the type strains of the phylogenetically closest species of the genus Marinobacterium revealed less than 70 % DNA-DNA relatedness, supporting the novel species status of the strain.
Abstract: A Gram-negative, aerobic bacterium, designated R-40509T, was isolated from mucus of the reef builder coral (Mussismilia hispida) located in the Sao Sebastiao Channel, Sao Paulo, Brazil. The strain was oxidase-positive and catalase-negative, and required Na+ for growth. Its phylogenetic position was in the genus Marinobacterium and the closest related species were Marinobacterium sediminicola, Marinobacterium maritimum and Marinobacterium stanieri; the isolate exhibited 16S rRNA gene sequence similarities of 97.5–98.0 % with the type strains of these species. 16S rRNA gene sequence similarities with other type strains of the genus Marinobacterium were below 96 %. DNA–DNA hybridizations between strain R-40509T and the type strains of the phylogenetically closest species of the genus Marinobacterium revealed less than 70 % DNA–DNA relatedness, supporting the novel species status of the strain. Phenotypic characterization revealed that the strain was able to grow at 15–42 °C and in medium containing up to 9 % NaCl. The isolate could be differentiated from phenotypically related species by several features, including its ability to utilize d-alanine, l-alanine, bromosuccinic acid, β-hydroxybutyric acid and α-ketovaleric acid, but not acetate or l-arabinose. It produced acetoin (Voges–Proskauer), but did not have esterase lipase (C8) or catalase activities. It possessed C18 : 1 ω7c (35 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 25 %) and C16 : 0 (22 %) as major cellular fatty acids. The DNA G+C content was 58.5 mol%. The name Marinobacterium coralli sp. nov. is proposed to accommodate this novel isolate; the type strain is R-40509T (=LMG 25435T =CAIM 1449T).

16 citations

Network Information
Related Topics (5)
Cohnella thermotolerans

12 papers, 396 citations

93% related
Tenacibaculum amylolyticum

4 papers, 426 citations

92% related
Levilinea saccharolytica

2 papers, 545 citations

92% related
EzTaxon Database

1 papers, 4.7K citations

91% related
Opitutus terrae

11 papers, 436 citations

91% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20211
20121
20111
20092
20071
20021