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Melibiose

About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.


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Journal ArticleDOI
TL;DR: Examination of the effects of various carbon sources on the cell wall and cytoplasmic activities of acid invertase, trehalase, maltase, cellobiase, melibiase and lactase found they could not be correlated with the growth-promoting activity of their substrates.
Abstract: The capacity of various carbohydrates to support growth of Japanese morning-glory callus in the dark over a 14-day period was examined. Sucrose was the most effective compound, but glucose, fructose, trehalose, maltose, cellobiose, raffinose and soluble starch supported significant growth. The callus remained alive in the presence of inulin, mannitol, inositol, methyl-α-glucoside and glycerol; while cells grown on galactose, mannose, sorbose, xylose, arabinose, melibiose, lactose, dextran, carboxy-methyl cellulose, sorbitol, galactitol, ethylene glycol were necrotic. Examination of the effects of these various carbon sources on the cell wall and cytoplasmic activities of acid invertase, trehalase, maltase, cellobiase, melibiase and lactase could not be correlated with the growth-promoting activity of their substrates. Extracellular hydrolysis of sucrose, trehalose, maltose, cellobiose, lactose, raffinose, and inulin occurred as a consequence of the presence of cell wall hydrolases in the morning-glory callus, and hydrolytic products could be detected in the medium.

11 citations

Journal ArticleDOI
TL;DR: Most of the “heat-resistant” strains with attenuated sporulating abilities, however, could sporulate well when raffinose or melibiose was the only carbohydrate present in a test medium.
Abstract: SUMMARY Isolates of Clostridium perfringens obtained from soil samples heated at 100°C and from samples heated at less than 100°C exhibited markedly different fermentation properties in tests with inositol, salicin, cellobiose, arbutin and aesculin. None of the C. perfringens isolates obtained from samples heated at 100°C for various lengths of time could ferment inositol, whereas all of the isolates obtained from unheated samples could ferment this sugar. The longer the preheating period at 100°C, the greater the number of aberrant isolates that attacked salicin, cellobiose, aesculin and arbutin, and the weaker were the sporulating abilities of these isolates. Most of the “heat-resistant” strains with attenuated sporulating abilities, however, could sporulate well when raffinose or melibiose was the only carbohydrate present in a test medium.

11 citations

Journal ArticleDOI
TL;DR: It is suggested that for cultures obtained during analytical procedures the reaction in litmus milk and the fermentation of melezitose and melibiose may be employed to distinguish between contamination representing recent pollution of human origin and the presence of S. faecalis as a member of the microflora of plants.
Abstract: More than 90% of 1618 cultures of Streptococcus faecalis obtained from plants, wild animals, and insects produced a soft, reduced, rennet-like curd which underwent stratiform digestion in litmus milk, or else produced no reaction. Cultures of human origin produced a reduced, hard, acidic curd which sometimes was followed by acid-proteolytic digestion. Ten percent of the cultures commensal in nature fermented lactose in litmus milk to produce the hard, acidic curd which sometimes underwent acid-proteolytic digestion. One-third of this group of organisms failed to follow the typical pattern of fermentation by S. faecalis of human origin, that is fermentation of melezitose but not of melibiose. It is suggested that for cultures obtained during analytical procedures the reaction in litmus milk and the fermentation of melezitose and melibiose may be employed to distinguish between contamination representing recent pollution of human origin and the presence of S. faecalis as a member of the microflora of plants...

11 citations

Journal ArticleDOI
TL;DR: Results indicated that TAG, rather than LacNAc, is a ligand for BHL‐14 on N‐linked oligosaccharide chains of glycoproteins.
Abstract: Preference for the β-anomer of galactose attributed to the bovine heart 14 kDa galectin-1 (BHL-14) was re-examined using natural glycoproteins and artificially glycosylated proteins as ligands. Endogenous glycoproteins co-purified with BHL-14 during its affinity chromatographic isolation contained oligosaccharides bearing terminal α-linked galactose (TAG) moieties and were superior even to laminin as ligands for homogeneous BHL-14 obtained by high pressure liquid chromatography. Artificially glycosylated proteins prepared by covalent attachment of melibiose to proteins and containing TAG moieties were ligands for BHL-14, unlike their lactose counterparts which contained β-linked galactose. Enzymatic removal of TAG moieties from the following glycoproteins abolished their recognition by BHL-14: (i) endogenous glycoproteins co-purified with BHL-14; (ii) mouse laminin; and (iii) bovine heart glycoproteins recognized by peanut agglutinin. Modification of TAG in laminin using galactose oxidase also rendered the glycoprotein inert towards BHL-14. Desialylation of human IgG, bovine thyroglobulin or laminin failed to increase the affinity of BHL-14 for these glycoproteins. Since removal of TAG or of sialic acid moiety exposed LacNAc (Gal β14 GlcNAc) in these glycoproteins, these results indicated that TAG, rather than LacNAc, is a ligand for BHL-14 on N-linked oligosaccharide chains of glycoproteins. Ready recognition of human IgA and jacalin-binding human plasma glycoproteins and non-recognition of human IgG suggested that T antigen (Galβ13 GalNAc) may also be ligand for galectin-1. Copyright © 2002 John Wiley & Sons, Ltd.

11 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20236
202212
202112
202017
201913
201816