Topic
Melibiose
About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.
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TL;DR: A natural lectin from the seed of peanut Arachis hypogaea was purified by singlestep affinity chromatography using galactoside-coupled agarose and showed a strong affinity for human A/B/O erythrocytes by neuraminidase treated and Agglutinating activity of PN-L was inhibited by lactose, raffinose, melibiose and D-galactose.
Abstract: A natural lectin from the seed of peanut Arachis hypogaea was purified by singlestep affinity chromatography using galactoside-coupled agarose. This lectin was named PN-L and its inactive form had a molecular mass estimate of 29 kDa. The lectin PN-L was detected for agglutinating activity, glycoinhibiting action and thermostability. The influence of pH on those activities was also tested. The results showed that PN-L could not agglutinate three kinds of human erythrocytes. But it showed a strong affinity for human A/B/O erythrocytes (RBC) by neuraminidase treated. Agglutinating activity of PN-L to neuraminidase treated human O erythrocytes was inhibited by lactose , raffinose, melibiose and D-galactose. The agglutinating activity of peanut seed lectin was inhibited at temperatures greater than 55 and at a pH less than 5 or greater than 11.
2 citations
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TL;DR: In this article, a novel invertase named InvDz13 was screened and purified from Microbacterium trichothecenolyticum and characterized, which had a specific activity of 229 U/mg toward raffinose at pH 6.5-7.5 and 25-40°C.
Abstract: The hydrolyzation of raffinose into melibiose by using invertases under mild conditions improves the nutritional value of soybean products. However, this strategy has received little attention because a suitable invertase remains lacking. In this study, a novel invertase named InvDz13 was screened and purified from Microbacterium trichothecenolyticum and characterized. InvDz13 was one of the invertases with the highest specific activity toward raffinose. Specifically, it had a specific activity of 229 U/mg toward raffinose at pH 6.5 and 35°C. InvDz13 retained more than 80% of its maximum activity at pH 5.5-7.5 and 25-40°C and was resistant to or stimulated by most cations that presented in soymilk. In soymilk treated with InvDz13 under mild conditions, melibiose concentration increased from 3.1 ± 0.2 to 6.1 ± 0.1 mM due to raffinose hydrolyzation by InvDz13. Furthermore, the prebiotic property of InvDz13-treated soymilk was investigated via in vitro fermentation by human gut microbiota. Results showed that InvDz13 treatment increased the proportion of the beneficial bacteria Bifidobacterium and Lactobacillus by 1.6- and 3.7-fold, respectively. By contrast, the populations of Escherichia and Collinsella decreased by 1.8- and 11.7-fold, respectively. Thus, our results proved that the enzymatic hydrolysis of raffinose in soymilk with InvDz13 was practicable and might be an alternative approach to improving the nutritional value of soymilk.
2 citations
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TL;DR: Under recommendations 3–4, rule 42 of the international code of nomenclature of bacteria, the name Clostridium hobsonii is proposed for U311, a species of biotechnological importance, in honor of P. Hobson B.Sc.
2 citations
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TL;DR: It is suggested that β-galactosidase, when melibiose-induced, is more stable than the average cellular proteins, and that alterations in its turnover may be involved in the mechanism of its induction.
2 citations
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TL;DR: The indium-mediated allylation reaction has been applied to melibiose and yielded the unprotected elongated disaccharides in the equilibrium of the pyranoid as well as furanoid isomers in both anomeric forms, respectively.
Abstract: The indium-mediated allylation reaction has been applied to melibiose, a disaccharidic substrate. This elongation methodology allows for a short, efficient and diastereoselective approach towards complex glycosylated carbohydrate structures. The stereochemical outcome of the key intermediates, allylated disaccharides, has been determined by X-ray analysis. Ozonolysis of the introduced double bond yielded the unprotected elongated disaccharides in the equilibrium of the pyranoid as well as furanoid isomers in both anomeric forms, respectively. Per-O-acetylation has been performed to facilitate separation of the isomeric mixture for structural identification. The main product revealed to adopt a β-pyranoid form of the elongated unit at the reducing end of the disaccharide.
2 citations