Melibiose

About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.

First report of bacterial black spot of mango in iran

Abstract: During 2012, mango’s orchards in different regions of Hormozgan province of Iran were evaluated. Plants with bacterial black spot symptoms were collected and transferred to the laboratory. Samples were washed with running water for 3 minutes, split to small pieces in sterile distilled water and were maintained in the laboratory condition for an hour. The resulting suspension was cultured in NA medium and incubated at 28˚C. Colonies that appeared at 2-5 days were purified and tested. All isolates were gram negative, obligate aerobic and were able to grow on SPA medium. Strains were positive in hypersensitive reaction on geranium, catalase, levan production, starch hydrolysis, hydrolysis of tween 80, H2s production from pepton and Simmon citrate agar. All isolates showed alkali reaction on litmus milk. Isolates were negative in oxidase test, arginin hydrolysis, nitrate reduction and acetoin production. Strains were able to use arabinos, inositol, methionin, raffinose, melibiose, mannitol, mannose, sucrose, erythritol, adonitol, trihalose, salicine, dulcitol, inolin and galactose; whereas weren’t able to use sorbitol. Pathogenicity test on mango’ leaf, were evaluated as positive. Symptom on treated leaf was very similar to mango black spot disease. Polymerase chain reaction with two Xanthomonas specific primers (RS21, RS22) led to amplify 1Kbp fragment. Furthermore, the results of PCR product sequencing and blast search showed that these strains have 98 percent similarity to Xanthomonas citri pv. citri. On the basis of phenotypic features, pathogenicity tests and molecular results, strains identified as Xanthomonas citri pv. mangiferaeindicae. This is the first report of mango bacterial black spot caused by X.citri pv. mangiferaeindicae in Iran.

Characterization and Culture Optimization of an Glucosidase Inhibitor-producing Bacteria, Gluconobactor oxydans CK-2165

Abstract: Miglitol, a well-known therapeutic intervention agents for diabetes, exhibits competitive inhibitory activityagainst α-glucosidase and it is usually produced through three sequential steps including chemical and bioconversionprocesses. Gluconobactor oxydans ( G. oxydans ) belonging to acetic acid bacteria biologically, converts 1-deoxy-1 -(2-hydroxyethylamino)-D-glucitol (P1) into a key intermidiate, 6-(2-hydroxyetyl) amino-6-deoxy-α-L-sorbofuranose (P2) by incomplete oxidation. In this study, we identified and optimized fermentation conditions of CK-2165, that was selected in soil samples by comparing the bioconversion yield. CK-2165 strain was found to be closely relatedto G. oxydans based on the result of phylogenetic analysis using 16S rDNA sequence. Utilization of API 20 kits revealed that this strain could use glucose, mannose, inositol, sorbitol, rhamnose, sucrose, melibiose, amygdalin andarabinose as carbon sources. The culture conditions were optimized for industrial production and several important factors affecting bioconversion rate were also tested using mycelial cake. Cell harvested at the late-stationary phaseshowed the highest bioconversion yield and MgSO

Mutants of Citrobacter freundii That Transport and Utilize Melibiose

Abstract: We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha-galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not sodium ions, were found to be the coupling cations for melibiose (and methyl-beta-D-thiogalactoside) transport in the mutant cells.

Bacillus stearothermophilus var. calidolactis C953'ten α-Galaktosidaz Üretimi*

Abstract: An intracellular, thermostable a-galactosidase (α-D-galactoside galactohydrolase EC 3.2.1.22.) was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity, came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in various carbon sources, raffinose produced the highest α-galactosidase activity, followed by melibiose, D-galactose and molasses, and that in the medium with soybean meal extract the second highest level of activity occurred. Soybean oligosaccharides (raffinose-like sugars), because of their present low commercial value, could be of pratical use in the production of α-galactosidase.

Bacillus sp. ZE01 from oceans and method for generating cold temperature-amylase by utilizing same

Abstract: The invention provides bacillus sp. ZE01 with collection number of CGMCC NO.10375. The strain is gram-positive bacillus sp. A bacterial colony is milk white and has a dry surface, wrinkles and neat edges. Through VP, gelatinase and glucose oxidation experiments, the strain is positive. Through beta-galactosidase, arginine dihydrolase, lysine decarboxylase, ornithine decarboxylase, tryptophan deiminase and urease experiments, the strain is negative. The strain does not utilize citric acids, does not generate hydrogen sulfide or indole and does not utilize sucrose, arabinose, rhamnose, melibiose, mannitol, inositol, sorbitol or amygdalin. The growth temperature of the strain ranges from 15 DEG C to 45 DEG C, the growth pH of the strain ranges from 5 to 9 and the NaCl concentration for growth of the strain ranges from 0% to 8%. The invention also discloses a method for generating cold temperature-amylase by utilizing the strain ZE01. The cold temperature-amylase has extensive application prospects in the fields of feeds, environmental protection, and the like.