Topic
Melibiose
About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.
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TL;DR: Melibiose, lactose, d-Galactose, alpha-methyl-d-galactopyranoside, N-acetylneuraminic acid, raffinose, and inulin were capable of inhibiting its hemagglutinating activity, with melibiose being the most potent.
248 citations
TL;DR: Exopolysaccharide production by Lactobacillus casei CG11 was studied in basal minimum medium containing various carbon sources; lactose and galactose were the poorest carbon sources, and glucose was by far the most efficient carbon source.
Abstract: Exopolysaccharide production by Lactobacillus casei CG11 was studied in basal minimum medium containing various carbon sources (galactose, glucose, lactose, sucrose, maltose, melibiose) at concentrations of 2, 5, 10, and 20 g/liter. L. casei CG11 produced exopolysaccharides in basal minimum medium containing each of the sugars tested; lactose and galactose were the poorest carbon sources, and glucose was by far the most efficient carbon source. Sugar concentrations had a marked effect on polymer yield. Plasmid-cured Muc- derivatives grew better in the presence of glucose and attained slightly higher populations than the wild-type strain. The values obtained with lactose were considerably lower for both growth and exopolysaccharide yield. The level of specific polymer production per cell obtained with glucose was distinctively lower for Muc- derivatives than for the Muc+ strain. The polymer produced by L. casei CG11 in the presence of glucose was different from that formed in the presence of lactose. The polysaccharide produced by L. casei CG11 in basal minimum medium containing 20 g of glucose per liter had an intrinsic viscosity of 1.13 dl/g. It was rich in glucose (76%), which was present mostly as 2- or 3-linked residues along with some 2,3 doubly substituted glucose units, and in rhamnose (21%), which was present as 2-linked or terminal rhamnose; traces of mannose and galactose were also present.
246 citations
TL;DR: A biochemical scheme was developed for the separation of Streptococcus mutans into 5 biotypes, a-e, which correlated with the recognized serotypes a--e, and the biotype identification of the Strep.
242 citations
TL;DR: It is demonstrated that the expected secondary amine linkages are formed with the ϵ-amino groups of lysine in synthetic glycoproteins prepared and compared with bovine serum albumin conjugates by amino acid analysis after acid hydrolysis.
219 citations
TL;DR: It was concluded that several starch-hydrolyzing strains of L. Beijerinck 1901 represent strains of a hitherto unrecognized species for which the name Lactobacillus amylovorus is proposed.
Abstract: Several starch-hydrolyzing strains of Lactobacillus Beijerinck 1901 were isolated from cattle waste-corn fermentations. The isolates were facultatively anaerobic, gram-positive, nonmotile, nonsporeforming, rod-shaped organisms that grew singly and in short chains, produced lactic acid and small amounts of acetic acid but no gas from glucose, and did not exhibit oxidase-, catalase-, or nitrate-reducing activities. Growth occurred at 45°C but not at 15°C. dl-Lactic acid was synthesized from glucose. An extracellular amylolytic enzyme was formed. The organisms fermented amygdalin, cellobiose, esculin, fructose, galactose, glucose, maltose, mannose, salicin, starch, sucrose, and trehalose. Some strains fermented mannitol and lactose. Arabinose, gluconate, melezitose, melibiose, raffinose, rham-nose, ribose, and xylose were not fermented. The average guanine-plus-cytosine content of the deoxyribonucleic acid was 40.4 mol%. Reassociation values of 15% or less were obtained with the deoxyribonucleic acids of the starch hydrolyzers and those of the type strains of Lactobacillus acidophilus (Moro) Hansen and Mocquot 1970; Lactobacillus jensenii Gasser, Mandel, and Rogosa 1970; and Lactobacillus leichmanii (Henneberg) Bergey et al. 1923. The new species was differentiated from the three recognized species on the basis of starch fermentation, vitamin requirements, guanine-plus-cytosine contents, and stereoisomerism of lactic acid produced. It was concluded that these organisms represent strains of a hitherto unrecognized species for which the name Lactobacillus amylovorus is proposed. The type strain of L. amylovorus is NRRL B-4540.
201 citations