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Melibiose

About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.


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Journal ArticleDOI
TL;DR: The carbohydrate analyzer used in this experimental work should become a useful tool in the clinical laboratory and the lower limit of detectable sugar in physiologic fluids is in the 1-µg range.
Abstract: Automated carbohydrate analysis can be useful clinically in the research laboratory as an aid in understanding the fundamental role of carbohydrates in metabolism, including their pathologic significance. An analytic system being developed at our laboratory utilizes an automated carbohydrate analyzer to chromatograph borated physiologic fluids while using strongly basic anion-exchange resin. The eluted carbohydrates are detected by sulfuric acid-phenol colorimetry. The carbohydrate analyzer used in this experimental work should become a useful tool in the clinical laboratory. Normal and diabetic human urine and blood serum have been chromatographed and significant differences established. As many as 38 peaks have been observed in the complex urine chromatograms. Using cochromatographic technics, 14 peaks have been tentatively identified as sucrose, raffinose, N-acetylglucosamine, maltose, lactose, ribose, fructose, arabinose, fucose, galactose, xylose, mannoheptulose, glucose, and glucose-1-phosphate. All except fucose have been quantified. Blood serum chromatograms consist of a major glucose peak and several smaller peaks indicating traces of other sugars. Melibiose has been used as an internal standard in chromatograms to determine recovery, resolution, and reproducibility. With the present technic, the lower limit of detectable sugar in physiologic fluids is in the 1-µg. range.

47 citations

Journal ArticleDOI
TL;DR: This paper reports some biochemical characteristics of a second L-proline transport system in Salmonella typhimurium and finds that it is an active transport system with an apparent Km for L- Proline of 3 x 10(-4) M and a strict specificity for L -proline and some of its analogs.
Abstract: This paper reports some biochemical characteristics of a second L-proline transport system in Salmonella typhimurium. In the accompanying paper, R. Menzel and J. Roth (J. Bacteriol. 141:1064--1070, 1980) have identified this system by showing that it is inactivated by mutations at the locus proP. We have found that it is an active transport system with an apparent Km for L-proline of 3 x 10(-4) M and a strict specificity for L-proline and some of its analogs. Unlike the L-proline transport system encoded in putP, this second system is induced by amino acid limitation.

47 citations

Journal ArticleDOI
TL;DR: To the authors' knowledge, this is the first report of gene cloning for Penicillium α-galactosidase belonging to family 36 of glycosyl hydrolases and expression in Pichia pastoris.

47 citations

Journal ArticleDOI
H Hama1, T H Wilson1
TL;DR: There is no universal Na+ recognition site for all the sugar substrates, instead different parts of the carrier seem to participate in cation recognition for different sugar substrate.

47 citations

Journal ArticleDOI
TL;DR: Observations suggest that the negatively-charged Asp55 residue, expected to be within the membrane embedded domain near the NH2 extremity of mel permease, is in or near the Na(+)-binding site and that the cation and sugar binding sites may be overlapping.

47 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20236
202212
202112
202017
201913
201816