Topic
Melibiose
About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.
Papers published on a yearly basis
Papers
More filters
TL;DR: Yeast strains belonging to 5 species gained the ability to ferment galactose, maltose, sucrose, raffinose and melibiose during cultivation in selective media, indicating that the saltants are stable in this medium.
Abstract: The acquisition of the ability to ferment galactose, maltose, sucrose, raffinose and melibiose by yeast strains of the genus Saccharomyces was investigated During cultivation in selective media 11 strains belonging to 5 species gained the ability to ferment one or several of these sugars De-adaptation was not usually observed after cultivation in glucose medium, indicating that the saltants are stable in this medium
34 citations
TL;DR: Results of other experiments provided evidence that raffinose was partly converted to 6''-carboxyraffinose when treated with galactose oxidase and catalase, and indicate that galactOSE oxidase not only converts the C-6 hydroxymethyl group of Galactose to an aldehyde group, but also catalyzes further oxidation to the carboxyl group.
34 citations
TL;DR: A galactose oxidase from Fusarium sambucinum was cloned and expressed in E. coli and it could be purified with a one step affinity chromatography step and biochemical characteristics of the enzyme are comparable to galactOSE oxidases.
34 citations
TL;DR: Azotobacter salinestris sp.
Abstract: Azotobacter salinestris sp. nov. was isolated from slightly saline soils of Western Canada, where strains of this species accounted for ≤5% of the aerobic nitrogen-fixing isolates. Also, one isolate was obtained from a saline Egyptian soil. These bacteria shared many physiological traits with Azotobacter chroococcum, but were absolutely dependent on Na+ ions for growth and frequently used melibiose as a carbon and energy source. Aerobically grown cells were melanized dark brown to black; the cells were large, gram negative, oval with pointed ends, and motile by means of peritrichous flagella and formed pairs and chains of six to eight cells during active growth. Capsule production was variable. Nitrogen fixation by A. salinestris occurred optimally at 35°C in the presence of molybdate or vanadate ions in a microaerophilic, aeroadaptive manner. The cells were very sensitive to H2O2 and catalase negative. However, a single weak catalase electromorph was observed in cell extracts. This contrasted with a very active catalase represented by multiple electromorphs in A. chroococcum. Iron was absolutely required for growth and aeroadaptation. Other growth-promoting substrates included fructose, galactose, glucose, mannitol, starch, and sucrose. Acid was formed from growth-promoting sugars and also from the non-growth-promoting substrates arabinose, cellobiose, lactose, mannose, rhamnose, and xylose. Incubation in the presence of increased NaCl concentrations promoted acidification of the culture to inhibitory levels, and sufficient acid was released from nitrogen-fixing cells in the presence of 1.0 to 1.5% NaCl to solubilize CaCO3 suspended in solid medium. The cells grew well in marine broth alone, producing an alkaline reaction. An acid reaction was produced both oxidatively and fermentatively in marine broth containing glucose. Nitrate was used in an assimilatory fashion, and there was no evidence of NO2- or N2 formation. The type strain is strain 184 (=ATCC 49674), which was isolated from soils of Alberta, Canada.
34 citations
TL;DR: A bifunctional enzyme from the human intestinal bacterium Ruminococcus gnavus E1, α-galactosidase/sucrose kinase (AgaSK), which produces sucrose-6-phosphate directly from raffinose points toward a novel glycolytic pathway in bacteria.
34 citations