Topic
Melibiose
About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.
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TL;DR: The optimum hydrogen-ion concentration for the action of invertase from the alimentary canal of the pea aphid, Acyrthosiphon pisum, was determined as pH 6·2 at 37°C; the enzyme hydrolysed sucrose, trehalose, melizitose, turanose, and maltose but did not hydrolyse melibiose or raffinose; it is therefore an α-glucosidase.
26 citations
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TL;DR: Penicillium oxalicum SO α-galactosidase demonstrated weak hydrolysing activity but a high rate of transglycosylation in the reaction with melibiose, where the major product was 6-α-Galactosyl Melibiose as mentioned in this paper.
25 citations
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TL;DR: In this article, a Galactose oxidase (Gal-OD) was placed between two dialysis membranes and tightened to a silver-platinum electrode, after its immobilisation in gelatine, and the activity of the enzyme electrode was increased greatly through applying potassium ferricyanide and copper chloride.
25 citations
01 Jan 1995
TL;DR: The authors 0.45 0.48 0.33 0.31 SR 0.36 0.41 0.39 0.24 0.10 0.11 SMeg 0 0.25 0.15 0.12 0.
Abstract: :SD 0.45 0.45 0.48 0.45 0.42 0.44 SS 0.40 0.39 0.41 0.40 0.39 0.39 SM 0.36 0.36 0.36 0.35 0.33 0.31 SR 0.29 0.30 0.10 0.12 0.11 0.11 SMeg 0 0.25 0 0.15 0 0.24
25 citations
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TL;DR: A glucose‐dependent hemagglutinin, present in the serum of an apparently healthy donor, is shown to be a polyclonal IgM immunoglobulin with specificity for β‐D‐glucopyranose configuration, and antibodies are present in sera of all normal adults against erythrocytes coated with melibiose and N‐acetylmannosamine.
Abstract: A glucose-dependent hemagglutinin, present in the serum of an apparently healthy donor, is shown to be a polyclonal IgM immunoglobulin with specificity for beta-D-glucopyranose configuration. The antibody did not agglutinate erythrocytes coated with hexoses differing from glucose at C2, C3, or C4 positions or lacking the primary alcohol group at C6 position. The hemagglutination reaction, quantitated in a continuous flow system, was inhibited by the addition of 6-deoxyglucose, 5-thioglucose, 2-deoxyglucose, 2-aminoglucose or 3-0 methylglucose. D-glucose, in beta- but not in alpha-configuration, attached by a glycosidic bond to another glucose or to an aglycone, was also inhibitory. A cross-reactivity was demonstrated between glucose and 6-deoxyglucose by antibody absorption and elution techniques. The donor's serum contained independent antibodies that reacted with erythrocytes coated with melibiose, gentiobiose, cellobiose, or N-acetylmannosamine. Further investigation revealed that antibodies are present in sera of all normal adults against erythrocytes coated with melibiose and N-acetylmannosamine and with high frequency against erythrocytes coated with gentiobiose, L-rhamnose, cellobiose, D-mannose, lactose, or D-galactose.
25 citations