Topic
Melibiose
About: Melibiose is a research topic. Over the lifetime, 1002 publications have been published within this topic receiving 27300 citations. The topic is also known as: Melibiose.
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TL;DR: It is suggested that the PCMBS-sensitive sites and charged residues assigned to helix 2 form a cluster of amino acids that map in three rows with each row comprised of every fourth residue and suggest that these residues line the path of melibiose and its associated cation through the carrier.
22 citations
TL;DR: The enzyme hydrolyzed p-nitrophenyl-α-D-galactopyroside, raffinose, stachyose but not melibiose and galactomanno-oligosaccharides, indicating that this enzyme recognizes not only the galactose moiety but also other substrates.
Abstract: The α-galactosidase gene of Streptomyces coelicolor A3(2) was cloned, expressed in Escherichia coli and characterized. It consisted of 1497 nucleotides encoding a protein of 499 amino acids with a predicted molecular weight of 57,385. The observed homology between the deduced amino acid sequences of the enzyme and α-galactosidase from Thermus thermophilus was over 40%. The α-galactosidase gene was assigned to family 36 of the glycosyl hydrolases. The enzyme purified from recombinant E. coli showed optimal activity at 40 °C and pH 7. The enzyme hydrolyzed p-nitrophenyl-α-D-galactopyroside, raffinose, stachyose but not melibiose and galactomanno-oligosaccharides, indicating that this enzyme recognizes not only the galactose moiety but also other substrates.
22 citations
Journal Article•
TL;DR: Sucrose, raffinose, stachyose, melibiose, and maltose were identified in the berries, leaves, and phloem of eight varieties of Vitis vinifera by paper chromatography, radioautography, and enzymatic techniques as discussed by the authors.
Abstract: Sucrose, raffinose, stachyose, melibiose, and maltose were identified in the berries, leaves, and phloem of eight varieties of Vitis vinifera by paper chromatography, radioautography, and enzymatic techniques. Galactose was also found in some berry samples. Sucrose and raffinose were present in berries at all stages of fruit development except the first 2 to 3 weeks after berry set. These sugars were present in low concentrations (less than 0.05%) until after the total soluble solids reached 10 to 18°B; they were at their highest level during berry maturity, and generally decreased slightly during overmaturity. The maximum concentrations of sucrose and raffinose in the different varieties respectively varied from 0.019 to 0.18% and from 0.015 to 0.34%. The concentration of sucrose and of raffinose in mature `Thompson Seedless9 and 9Semillon9 berries was greater in the basal part than in the middle or tip sections.
22 citations
TL;DR: It was concluded that there is some type of physiological interaction between His-322 and Glu-325 and that the complete loss of transport activity due to the removal of the negative charge at 325 can be partially compensated for by the introduction of a newnegative charge at 322.
22 citations
TL;DR: The data are consistent with the hypothesis that for normal growth of inducible cells of lactose or melibiose, carrier-mediated entry (facilitated diffusion) of substrates is inadequate; the membrane carriers must also be capable of accumulating the sugars.
22 citations