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Membrane lipids

About: Membrane lipids is a research topic. Over the lifetime, 6910 publications have been published within this topic receiving 409561 citations.


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Journal ArticleDOI
TL;DR: This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what isknown of domain formation in cell membranes.
Abstract: Views of how cell membranes are organized are presently changing. The lipid bilayer that constitutes these membranes is no longer understood to be a homogeneous fluid. Instead, lipid assemblies, termed rafts, have been introduced to provide fluid platforms that segregate membrane components and dynamically compartmentalize membranes. These assemblies are thought to be composed mainly of sphingolipids and cholesterol in the outer leaflet, somehow connected to domains of unknown composition in the inner leaflet. Specific classes of proteins are associated with the rafts. This review critically analyzes what is known of phase behavior and liquid-liquid immiscibility in model systems and compares these data with what is known of domain formation in cell membranes.

1,615 citations

Journal ArticleDOI
TL;DR: Membrane fatty acid composition, phospholipid composition, and cholesterol content can be modified in many different kinds of intact mammalian cells, and many of the functional responses probably are caused directly by the membrane lipid structural changes, which affect either bulk lipid fluidity or specific lipid domains.

1,437 citations

Journal ArticleDOI
26 Feb 2009-Nature
TL;DR: The ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells is demonstrated.
Abstract: Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events. Although several experiments indicate their existence, lipid nanodomains ('rafts') remain controversial owing to the lack of suitable detection techniques in living cells. The controversy is reflected in their putative size of 5-200 nm, spanning the range between the extent of a protein complex and the resolution limit of optical microscopy. Here we demonstrate the ability of stimulated emission depletion (STED) far-field fluorescence nanoscopy to detect single diffusing (lipid) molecules in nanosized areas in the plasma membrane of living cells. Tuning of the probed area to spot sizes approximately 70-fold below the diffraction barrier reveals that unlike phosphoglycerolipids, sphingolipids and glycosylphosphatidylinositol-anchored proteins are transiently ( approximately 10-20 ms) trapped in cholesterol-mediated molecular complexes dwelling within <20-nm diameter areas. The non-invasive optical recording of molecular time traces and fluctuation data in tunable nanoscale domains is a powerful new approach to study the dynamics of biomolecules in living cells.

1,434 citations

Journal ArticleDOI
TL;DR: It was found that compositional variation results in the biosynthesis of phospholipids that have identical viscosities at the temperature of growth of the cells, and this "homeoviscous adaptation" can also be observed in E. coli membrane preparations.
Abstract: E. coli incorporates increasing proportions of saturated and long-chain fatty acids into phospholipids as growth temperature is increased. It was found that this compositional variation results in the biosynthesis of phospholipids that have identical viscosities at the temperature of growth of the cells. This “homeoviscous adaptation” can also be observed in E. coli membrane preparations. Viscosities were determined by use of the electron spin resonance spin-label technique.

1,393 citations

Journal ArticleDOI
TL;DR: Lipid biochemistry has remained a fairly esoteric branch of molecular cell biology, but this situation is now gradually changing with the discovery of phosphoinositide involvement in signal transduction.
Abstract: One of the challenges of contemporary cell biology is to unravel how the molecular composition of the different cellular compartments is generated and maintained during the cell cycle. In animal cells most of the efforts have been directed toward the study of how newly synthesized proteins are transported to their correct cellular destinations, whereas the lipids, which make up the framework of the membranes in the cell, have been given much less attention. Lipid biochemistry has remained a fairly esoteric branch of molecular cell biology. This situation is now gradually changing with the discovery of phosphoinositide involvement in signal transduction (Ber- ridge,

1,378 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202319
202239
2021146
2020170
2019121
2018162