Topic
Methanogen
About: Methanogen is a research topic. Over the lifetime, 1146 publications have been published within this topic receiving 48254 citations.
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TL;DR: The results suggested that syntrophic biodegradation of organic substrates by the H2‐producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH4 production.
Abstract: Summary
In a deep aquifer associated with an accretionary prism, significant methane (CH4) is produced by a subterranean microbial community. Here, we developed bioreactors for producing CH4 and hydrogen (H2) using anaerobic groundwater collected from the deep aquifer. To generate CH4, the anaerobic groundwater amended with organic substrates was incubated in the bioreactor. At first, H2 was detected and accumulated in the gas phase of the bioreactor. After the H2 decreased, rapid CH4 production was observed. Phylogenetic analysis targeting 16S rRNA genes revealed that the H2-producing fermentative bacterium and hydrogenotrophic methanogen were predominant in the reactor. The results suggested that syntrophic biodegradation of organic substrates by the H2-producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH4 production. For H2 production, the anaerobic groundwater, amended with organic substrates and an inhibitor of methanogens (2-bromoethanesulfonate), was incubated in a bioreactor. After incubation for 24 h, H2 was detected from the gas phase of the bioreactor and accumulated. Bacterial 16S rRNA gene analysis suggested the dominance of the H2-producing fermentative bacterium in the reactor. Our study demonstrated a simple and rapid CH4 and H2 production utilizing anaerobic groundwater containing an active subterranean microbial community.
8 citations
TL;DR: Both the leaf blade and stem pith of corn stover have the potential to produce methane in a simple co-culture of an anaerobic fungus and methanogen.
Abstract: To determine ways to improve the utilization of corn stover, this study investigated methane production from different parts of corn stover using a simple co-culture of an anaerobic fungus (Pecoramyces species) and methanogen (Methanobrevibacter species). The simple co-culture was incubated with the stem pith, leaf blade, or stem bark of corn stover (as substrates) at 39°C for 72 h. The results showed that the stem bark had the lowest (P < 0.05) digestibility (38.0 ± 1.36%) and neutral detergent solubles, that is, cell solubles (31.6 ± 0.45%), and the highest (P < 0.05) lignin content (4.8 ± 0.56%). The leaf blade had a significantly higher methane conversion rate (56.6 ± 0.76 mL/g digested substrate) than the stem pith (49.2 ± 1.60 mL/g digested substrate), even though they showed similar levels of methane production (42.4 ± 1.0 mL and 40.9 ± 1.35 mL, respectively). Both the leaf blade and stem pith of corn stover have the potential to produce methane in a simple co-culture of an anaerobic fungus and methanogen.
8 citations
TL;DR: In experiments where liquid fractions from the soil simulant and water mixtures were used in place of the buffer, two out of three of the species demonstrated significantly greater methane production compared to the buffer.
8 citations
TL;DR: In this article, the authors designed a laboratory experiment to evaluate microbial growth and secondary mineralization pathways in the presence and absence of microbial methanogenesis, where they used synchrotron X-ray absorption spectroscopic techniques to detect rare and microscale Fe-bearing solid-phase reaction products.
8 citations
TL;DR: In this paper, the impact of granular activated carbon (GAC) on anaerobic co-digestion of blackwater (BW, municipal toilet wastewater) and organic kitchen waste (KW) was studied under thermophilic (55°C) temperatures.
7 citations