Topic
Methanogen
About: Methanogen is a research topic. Over the lifetime, 1146 publications have been published within this topic receiving 48254 citations.
Papers published on a yearly basis
Papers
More filters
Journal Article•
TL;DR: In this article, the distribution of archaeol, the core membrane structure components, is analyzed in the sediment from two wells in the Sanhu depression Qaidam basin, and the results showed a good relationship between methanogen and salinity.
Abstract: Biogenic gas is the product of methanogen in anaoxic environment.Its formation and occurrence is controlled by the distribution of methanogen.The present method in studying the distribution of methanogen is MPN(numbers of methanogen).Because methanogen is strict with its environment,especially with the occurrence of oxygen,trivial change should disturb the bacterial collects and make the number different from the reality.Otherwise,collection of the fresh groundwater is difficult for us.All those make MPN turn to be hard to be dealed with.The concentration of some special biomarkers of methanogen,especially its core membrane components,could reflect the distribution of methanogen for a time.This method is simple and easy to operate,could make up the deficiency.In this paper,the distribution of archaeol,the core membrane structure components,is analyzed in the sediment from two wells in the Sanhu depression Qaidam basin.The results showed a good relationship between methanogen and salinity.In high salt area,the activity of methanogens was depressed in the shallow(above 1000m),and then turned to active up to 2000m.While in the surrounding area like Sebei 1 area,the salinity of sediment is much lower,the depression of methanogen in the shallow is slight that make methanogen mainly concentrate above 1000m and is low below 1000m.In the final,combined with the sediment palaeoenvironment and salinity of the sediment,the activation of methanogen in Sanhu depression is characterized.
2 citations
TL;DR: Wang et al. as mentioned in this paper conducted a three-month indoor mesocosm experiment to ascertain the effects of thifluzamide (0.05, 0.5, and 5 mg kg-1 soil) on bacterial and archaeal community structure and on the abundance of methanogen genes using two typical paddy soils.
2 citations
TL;DR: In this article , the influence of organic load on biogas production from food waste and the response of microbial community was investigated and it was shown that high organic load led to decrease of hydrolysis bacteria and methanogen while it helped to increase the relative abundance of hydrogen-producing bacteria.
2 citations
TL;DR: A novel methodology based on high-pressure microfluidics to rapidly perform temperature-based phenotyping of microbial strains from deep-sea environments is presented, overcoming the conventional limitations of large-scale batch metal reactors to conduct fast screening investigations.
Abstract: Here, we present a novel methodology based on high-pressure microfluidics to rapidly perform temperature-based phenotyping of microbial strains from deep-sea environments. The main advantage concerns the multiple on-chip temperature conditions that can be achieved in a single experiment at pressures representative of the deep-sea, overcoming the conventional limitations of large-scale batch metal reactors to conduct fast screening investigations. We monitored the growth of the model strain Thermococcus barophilus over 40 temperature and pressure conditions, without any decompression, in only 1 week, whereas it takes weeks or months with conventional approaches. The results are later compared with data from the literature. An additional example is also shown for a hydrogenotrophic methanogen strain (Methanothermococcus thermolithotrophicus), demonstrating the robustness of the methodology. These microfluidic tools can be used in laboratories to accelerate characterizations of new isolated species, changing the widely accepted paradigm that high-pressure microbiology experiments are time-consuming.
2 citations
TL;DR: The catabolic pathways of glycerol conversion were identified by genome analysis of the two Thermoanaerobacter strains and NADH and reduced ferredoxin formed in the pathway are linked to proton reduction, which becomes thermodynamically favourable when the hydrogen partial pressure is kept low by the hydrogenotrophic methanogenic partner.
Abstract: Glycerol-rich waste streams produced by the biodiesel, bioethanol and oleochemical industries can be treated and valorized by anaerobic microbial communities to produce methane. As current knowledge of the microorganisms involved in thermophilic glycerol conversion to methane is scarce, thermophilic glycerol-degrading methanogenic communities were enriched. A co-culture of Thermoanaerobacter and Methanothermobacter species was obtained, pointing to a non-obligately syntrophic glycerol degradation. This hypothesis was further studied by incubating Thermoanaerobacter brockii subsp. finnii and T. wiegelii with glycerol (10 mM) in pure culture and with different hydrogenotrophic methanogens. The presence of the methanogen accelerated glycerol fermentation by the two Thermoanaerobacter strains up to 3.3 mM day-1 , corresponding to 12 times higher volumetric glycerol depletion rates in the methanogenic co-cultures than in the pure bacterial cultures. The catabolic pathways of glycerol conversion were identified by genome analysis of the two Thermoanaerobacter strains. NADH and reduced ferredoxin formed in the pathway are linked to proton reduction, which becomes thermodynamically favourable when the hydrogen partial pressure is kept low by the hydrogenotrophic methanogenic partner.
2 citations