Methanosarcina barkeri

About: Methanosarcina barkeri is a research topic. Over the lifetime, 703 publications have been published within this topic receiving 32151 citations.

Membranes of bacteria lacking peptidoglycan

Abstract: Bacteria lacking peptidoglycan come into four main categories: Mycoplasmas, bacterial L-forms, halobacteria and methanogenic bacteria. The last-named were only recently identified as falling in this class, when Kandier and Hippe [14] showed that the walls of Methanosarcina barken and some other species lacked muramic acid, glucosamine and D-glutamic acid. The structural component of the wall of M. barkeri consisted of galactosamine, uronic acids, glucose and a little galactose and thus resembled that of Halococcus morrhuae (see below) except that it was not sulphated. Further evidence has now indicated that many of the Methanobacteria have walls containing ‘pseudomurein’ (see Chapter 6) and that they may be considered as a group along with the Halobacteria, Thermosplasma and Sulfolobus to form a separate kingdom of prokaryotes designated Archaebacteria [66].

Sodium Ion Translocation and ATP Synthesis in Methanogens

Abstract: Methanogens are the only significant biological producers of methane A limited number of C(1) substrates, such as methanol, methylamines, methyl sulfate, formate, H(2)+CO(2) or CO, and acetate, serve as carbon and energy source During degradation of these compounds, a primary proton as well as a primary sodium ion gradient is established, which is a unique feature of methanogens This raises the question about the coupling ion for ATP synthesis by the unique A(1)A(o) ATP synthase Here, we describe how to analyze and determine the Na(+) dependence of two model methanogens, the hydrogenotrophic Methanothermobacter thermautotrophicus and the methylotrophic Methanosarcina barkeri Furthermore, the determination of important bioenergetic parameters like the ΔpH, ΔΨ, or the intracellular volume in M barkeri is described For the analyses of the A(1)A(O) ATP synthase, methods for measurement of ATP synthesis as well as ATP hydrolysis in Methanosarcina mazei Go1 are described

Presence of an unusual methanogenic bacterium in coal gasification waste.

Abstract: Methanogenic bacteria growing on a pilot-scale, anaerobic filter processing coal gasification waste were enriched in a mineral salts medium containing hydrogen and acetate as potential energy sources. Transfer of the enrichments to methanol medium resulted in the initial growth of a strain of Methanosarcina barkeri, but eventually small cocci became dominant. The cocci growing on methanol produced methane and exhibited the typical fluorescence of methanogenic bacteria. They grew in the presence of the cell wall synthesis-inhibiting antibiotics d-cycloserine, fosfomycin, penicillin G, and vancomycin as well as in the presence of kanamycin, an inhibitor of protein synthesis in eubacteria. The optimal growth temperature was 37°C, and the doubling time was 7.5 h. The strain lysed after reaching stationary phase. The bacterium grew poorly with hydrogen as the energy source and failed to grow on acetate. Morphologically, the coccus shared similarities with Methanosarcina sp. Cells were 1 μm wide, exhibited the typical thick cell wall and cross-wall formation, and formed tetrads. Packets and cysts were not formed. Images

Bactericide for generating methane and preparation technology thereof

Abstract: The invention relates to a microbial agent and a preparation technology thereof, belonging to the technical filed of agricultural production. In the preparation technology, Clostridium thermocellum, Clostridium beijerinckii, Methanocaldococcus jannaschii, Methanothermobacter wolfeii, Methanosarcina thermophila and Methanosarcina barkeri Schnellen are coordinated in mass ratio of 1-2:1-3:1:1:1:1-4, the total number of effective viable bacteria, the operation is carried out in an asepsis condition, and the product is kept is a dark place. To a house hold fermenting tank, a bactericide for generating methane is required to be added, the additive amount accounts for 0.1-0.2% of the feeding amount; to a semi-batch (semi-continuous) fermentation mode and a continuous fermentation mode, the additive amount accounts for 0.05-0.1% of the feeding amount. The bactericide for generating methane has wide adaptability to temperature, can generate methane continuously from 30 DEG C to 85 DEG C, and does not have strict requirements for organic matter materials. The technology can be used for household small-size methane tanks and large-scale industrial methane apparatuses to use.