Showing papers on "Michelia published in 2016"
TL;DR: A recent re-collection of the species is reported from four locations in the West Khasi Hills after a lapse of almost 100 years so as to develop effective strategies for its conservation.
Abstract: Magnolia lanuginosa (Wall.) Figlar & Noot. [= Michelia lanuginosa Wall.], a rare tree species of Meghalaya, is restricted to the West Khasi Hills District, Meghalaya. The species was considered to have become extinct from the state. The present paper reports a recent re-collection of the species from four locations in the West Khasi Hills after a lapse of almost 100 years. In addition, the population structure, regeneration status and the threat to the species are also discussed so as to develop effective strategies for its conservation.
11 citations
TL;DR: The chemical constituents of hydrodistilled essential oils from the leaves of four Magnoliaceae species were examined in this article, and the main compounds in Manglietia fordiana oils were δ-cadinene (18.0%), (E)-nerolidol (16.7%), α-copaene (12.8%), β-selinene (9.1%), and limonene (8.3%).
Abstract: The chemical constituents of hydrodistilled essential oils from the leaves of four Magnoliaceae species were examined. The main compounds in Manglietia fordiana oils were δ-cadinene (18.0%), (E)-nerolidol (16.7%), α-copaene (12.8%), α-selinene (9.1%), and limonene (8.3%); in Manglietia conifera oils they were β-caryophyllene (29.9%), α-humulene (7.4%), (E)-nerolidol (6.3%), and caryophyllene oxide (5.5%). The major constituents of Michelia mediocris oil were (E)-nerolidol (36.4%), δ-cadinene (10.9%), and β-selinene (7.2%), while in Michelia tonkinensis they were α-pinene (40.3%), β-phellandrene (7.6%), β-pinene (7.4%), and bicyclogermacrene (7.3%).
8 citations
TL;DR: In this article, the authors identified the chemical constituents of the Michelia figo from the stems of the plant and extracted four alkaloids from the stem using MeOH (5 L 3) at room temperature, and a MeOH extract (53.4 g) was obtained upon concentration under reduced pressure.
Abstract: Species belonging to the Michelia genus are arboreous plants, growing in temperate zone of oriental India, southern China, Malaysia, and Indonesia. The species more utilized is Michelia champaca: its cortex and seeds are used as febrifuge and tonic-aromatic, its roots are employed as emmenagogue, its leaves as astringent, its gemmae in the treatment of hemorrhage and its flowers and fruits are believed to possess curative properties in enteritis [1]. Less known species, such as Michelia figo, are used as ornamental plants and to obtain essences. [1]. Michelia figo is an evergreen medium shrub, commonly called banana shrub, because of the heavy, sweet fragrance banana scent of its purple flowers. The plant is also known in Indian folk medicine as a remedy against hypertension [2]. To further understand the chemotaxonomy of the Michelia species [3–11], M. figo was chosen for phytochemical investigation. The chemical constituents of this plant have not yet been reported. The compounds derived from the stem include four alkaloids, (6,7-dimethoxyisoquinolinyl)-(4 -methoxyphenyl)-methanone [12], corydaldine [13], N-methylcorydaldine [14], and liriodenine [15]; two steroids, 6 -hydroxystigmast-4-en-3-one [16] and 6 -hydroxystigmast-4,22-dien-3-one [16]; five benzenoids, p-hydroxybenzaldehyde [17], p-hydroxybenzoic acid [18], 3,4,5-trimethoxybenzoic acid [18], vanillin [18], and vanillic acid [18]. All of these compounds were found for the first time from this plant. The stems of M. figo Spreng. were collected from Chiayi County, Taiwan, in May 2011. Plant material was identified by Prof. Fu-Yuan Lu (Department of Forestry and Natural Resources, College of Agriculture, National Chiayi University). A voucher specimen (Michelia 5) was deposited in the School of Medical and Health Sciences, Fooyin University, Kaohsiung City, Taiwan. The air-dried stems of M. figo (2.3 kg) were extracted with MeOH (5 L 3) at room temperature, and a MeOH extract (53.4 g) was obtained upon concentration under reduced pressure. The MeOH extract, suspended in H2O (1 L), was partitioned with CH2Cl2 (3 L 4) to give fractions soluble in CH2Cl2 (40.5 g) and H2O (7.7 g). The CH2Cl2-soluble fraction was chromatographed over silica gel (900 g, 70–230 mesh) using n-hexane–EtOAc–MeOH mixtures as eluents to give five fractions. Part of fraction 2 (7.04 g) was subjected to silica gel chromatography eluting with n-hexane–EtOAc (50:1), enriched gradually with EtOAc, to furnish five fractions (2-1–2-5). Fraction 2-2 (2.51 g) was further purified on a silica gel column using n-hexane–EtOAc mixtures to obtain a mixture of 6 -hydroxystigmast-4-en-3-one and 6 -hydroxystigmast-4,22-dien3-one (14 mg). Part of fraction 3 (5.68 g) was subjected to silica gel chromatography eluting with n-hexane–EtOAc (40:1), enriched with EtOAc, to furnish three further fractions (3-1–3-3). Fraction 3-2 (2.11 g) was further purified on a silica gel column using CH2Cl2–MeOH mixtures to obtain corydaldine (5 mg) and N-methylcorydaldine (4 mg). Fraction 3-3 (2.18 g) was further purified on a silica gel column using CH2Cl2–MeOH mixtures to obtain liriodenine (17 mg) and (6,7-dimethoxyisoquinolinyl)-(4 -methoxyphenyl)-methanone (3 mg). Part of fraction 4 (7.65 g) was subjected to silica gel chromatography eluting with CH2Cl2–MeOH (40:1), enriched with MeOH, to furnish four fractions (4-1–4-4). Fraction 4-2
2 citations
Patent•
15 Jun 2016
TL;DR: In this paper, a michelia alba tissue culture method is described, which comprises the following steps: taking a micro-alba stem tip as an explant, disinfecting, inoculating to a bud induction culture medium, and culturing for 6 days to 10 days until new buds grow; transferring into a rooting culture medium andculturing for 13 days to 15 days until roots of 1cm to 3cm grow.
Abstract: The invention discloses a michelia alba tissue culture method, relating to the technical field of tissue culture The michelia alba tissue culture method comprises the following steps: taking a michelia alba stem tip as an explant, disinfecting, inoculating to a bud induction culture medium, and culturing for 6 days to 10 days until new buds grow; transferring into a rooting culture medium, and culturing for 13 days to 15 days until roots of 1cm to 3cm grow; transferring into a strong seedling culture medium, and culturing for 10 days to 15 days until seedlings grow tall by 3cm to 4cm, and culturing by seedling hardening for 15 days to 20 days; transplanting to an open field for cultivation By using all the culture media, the obtained buds are strong and orderly, leaves are light green, the grown roots are great in amount, and the seedlings are thick, strong, light green and good in growth vigor; the planting period of michelia alba is shortened, and the planting of the michelia alba is easier to grasp; the method is a convenient, saving, quick and efficient michelia alba propagation method
1 citations
TL;DR: The present oil compositions were found to be different from the results obtained previously from the essential oils of Michelia species grown in Vietnam and other parts of the world.
Abstract: The chemical constituents of essential oils obtained from the hydrodistillation of the leaf and stem of Michelia foveolata Merr. et Dandy (Magnoliaceae) were being reported. The main compounds of the leaf essential oil are β-caryophyllene (37.1%) and bicyclogermacrene (23.3%). However, βcaryophyllene (26.4%), germacrene D (5.9%), trans-α-bergamotene (5.6%), δ-cadinene (5.5%), bicyclogermacrene (5.4%), bicycloelemene (5.3%), spathulenol (5.3%) and 3,7-guaiadiene (5.1%) were present in the stem. Aims: The aim of this study was to isolate essential oils from the leaf and stem bark of Michelia Original Research Article Dai et al.; JSRR, 10(6): 1-6, 2016; Article no.JSRR.25304 2 foveolata Merr. et Dandy (Magnoliaceae) and investigate the volatile constituents present therein. Study Design: The study involves the hydrodisitillation of essential oils from the air-dried plant materials and analysis of their chemical composition. Place and Duration of Study: The leaf and stem bark of M. foveolata were collected from Vũ Quang National Park, Ha Tĩnh Province Vietnam, in July 2014. Methodology: About 500 g of air-dried plant samples was shredded and their oils were obtained by separate hydrodistillation for 4 h at normal pressure, according to the Vietnamese Pharmacopoeia. The chemical constituents of the distilled oils were analyzed by means of gas chromatography-flame ionization detector (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Results: The main compounds of the leaf essential oil are β-caryophyllene (37.1%) and bicyclogermacrene (23.3%) while the stem bark contained large amounts of β-caryophyllene (26.4%), germacrene D (5.9%), trans-α-bergamotene (5.6%), δ-cadinene (5.5%), bicyclogermacrene (5.4%), bicycloelemene (5.3%), spathulenol (5.3%) and 3,7-guaiadiene (5.1%). Conclusion: The present oil compositions were found to be different from the results obtained previously from the essential oils of Michelia species grown in Vietnam and other parts of the world.
1 citations
Patent•
15 Jun 2016
TL;DR: In this article, a michelia alba callus induction method relating to the technical field of tissue culture is described, which comprises the following steps: by taking a microbe petal as an explant, disinfecting by 75percent alcohol, then transferring into a basic solution for soaking, and finally transferring into an MS culture medium for induction, wherein the culture medium is characterized in that an MS medium is taken as a basic culture medium, and 80gram/liter to 120gram /liter of 6-benzylamino adenine and 10gram/liters of
Abstract: The invention discloses a michelia alba callus induction method, relating to the technical field of tissue culture. The method comprises the following steps: by taking a michelia alba petal as an explant, disinfecting by 75percent alcohol, then transferring into a basic solution for soaking, and finally transferring into a culture medium for induction, wherein the culture medium is characterized in that an MS culture medium is taken as a basic culture medium, and 80gram/liter to 120gram/liter of 6-benzylamino adenine and 10gram/liter to 20gram/liter of lysine are added inside. The method improves the rooting percentage and rooting quality of michelia alba.
Patent•
25 May 2016
TL;DR: In this article, a preparation method of michelia figo tea is described, in which the wall-broken pollen of the corn flowers are mixed with a small amount of the longan polysaccharide, and then the mixture is packaged into a vacuum plastic food bag to obtain an ideal beverage material.
Abstract: The invention discloses a preparation method of michelia figo tea. The michelia figo tea is prepared from raw materials including tea flowers, michelia figo, corn flowers and longan polysaccharide, wherein the flowers above are treated by a grinding method to obtain wall-broken pollen of the flowers; the fruit longan is prepared into a dried product and the purified longan polysaccharide is separated from pulp of the dried product; the wall-broken pollen of the tea flowers, the wall-broken pollen of the michelia figo and the wall-broken pollen of the corn flowers are taken according to proper amount, and are mixed with small amount of the longan polysaccharide; and then the mixture is packaged into a vacuum plastic food bag to obtain an ideal beverage material which is nutritional, delicious and aromatic, and contains nutrients capable of being sufficiently absorbed by human bodies.