Michelia

About: Michelia is a research topic. Over the lifetime, 198 publications have been published within this topic receiving 1565 citations. The topic is also known as: Magnolia sect. Michelia.

Chemical Constituents of the Leaves of Michelia figo

Abstract: Species belonging to the genus Michelia are arboreous plants, growing in temperate zones of oriental India, southern China, Malaysia, and Indonesia. The species most utilized is Michelia champaca: its cortex and seeds are used as febrifuge and tonic-aromatic; the roots are employed as emmenagogue, the leaves as astringent, the gemmae in the treatment of hemorrhage, and the flowers and fruits are believed to possess curative properties in enteritis [1]. The less known species, M. figo, is used as ornamental plants and to obtain essences [1]. M. figo is an evergreen medium shrub, commonly called banana shrub, because of the heavy, sweet fragrant banana scent of its purple flowers. The plant is also known in Indian folk medicine as a remedy against hypertension [2]. To further understand the chemotaxonomy of the Michelia species [3–7], M. figo was chosen for phytochemical investigation. The chemical constituents of the leaves of this plant have not yet been reported. The compounds derived from the leaves include three alkaloids, (–)-nuciferine (1) [8], (–)-anonaine (2) [9], and N-methylcorydaldine (3) [10]; two steroids, -sitostenone (4) [9] and stigmasta-4,22-dien-3-one (5) [9]; four benzenoids, p-hydroxybenzaldehyde (6) [10], p-hydroxybenzoic acid (7) [11], methylparaben (8) [11], and vanillin (9) [11]; six chlorophylls, pheophytin a (10) [12], pheophorbide a (11) [12], pheophytin b (12) [13], pheophorbide b (13) [13], aristophyll-C (14) [14], 132-hydroxy-(132-S)pheophytin a (15) [15]; and one sesquiterpene lactone, 11,13-dehydrolanuginolide (16) [16]. All of these known compounds were obtained for the first time from the leaves of this plant and were identified by direct comparison with authentic samples (TLC, UV, IR, ESI-MS and NMR) and the literature [8–16]. The leaves of M. figo (Lour.) Spreng. were collected from Chiayi County, Taiwan, May 2011. Plant material was identified by Prof. Fu-Yuan Lu (Department of Forestry and Natural Resources, College of Agriculture, National Chiayi University). A voucher specimen (Michelia 5) was deposited in the School of Medicinal and Health Sciences, Fooyin University, Kaohsiung City, Taiwan. The air-dried leaves of M. figo (4.8 kg) were extracted with MeOH (6 L 4) at room temperature, and a MeOH extract (121.6 g) was obtained upon concentration under reduced pressure. The MeOH extract, suspended in H2O (1 L), was partitioned with CH2Cl2 (3 L 5) to give fractions soluble in CH2Cl2 (67.9 g) and H2O. The CH2Cl2-soluble fraction was chromatographed over silica gel (950 g, 70–230 mesh) using n-hexane–EtOAc–MeOH mixtures as eluents to give five fractions. Part of fraction 1 (8.24 g) was subjected to silica gel chromatography by eluting with n-hexane–EtOAc (60:1) and enriched gradually with EtOAc to furnish five fractions (1-1–1-5). Fraction 1-2 (3.11 g) was further purified on a silica gel column using n-hexane–EtOAc mixtures to obtain (12 mg) and 5 (6 mg). Part of fraction 2 (11.78 g) was subjected to silica gel chromatography by eluting with n-hexane–EtOAc (60:1) and enriched gradually with EtOAc to furnish five fractions (2-1–2-5). Fraction 2-1 (2.78 g) was further purified on a silica gel column using n-hexane–EtOAc mixtures to obtain 10 (3 mg) and 11 (4 mg). Fraction 2-2 (2.17 g) was further purified on a silica gel column using n-hexane–EtOAc mixtures to obtain 12 (1 mg) and 13 (2 mg). Fraction 2-3 (2.86 g) was further purified on a silica gel column using n-hexane–EtOAc mixtures to obtain 14 (13 mg) and 15 (9 mg). Fraction 2-4 (1.55 g) was further purified on a silica gel column using n-hexane–EtOAc mixtures to obtain 16 (18 mg). Part of fraction 3 (16.97 g) was subjected to silica gel chromatography by eluting with n-hexane–EtOAc (40:1) and enriched with EtOAc to furnish six further fractions (3-1–3-6). Fraction 3-3 (4.24 g) was further purified on a silica

Dedifferentiated guard cells in magnoliaceous leaves.

Abstract: Evidence has been obtained that guard cells and other epidermal cells as well as mesophyll cells undergo division during wound repair of mature leaves in 26 magnoliaceous taxa in the genera Kmeria, Elmerrillia, Magnolia, Manglietia, Michelia, Paramichelia, and Talauma. Division of epidermal cells is believed to be rare in mature leaves, and division of guard cells is particularly unusual in most species previously studied.

Comparison and Interpretation of Serological Data in the Magnoliaceae

Abstract: The precipitin reaction, as measured in liquid medium (Boyden method) and double-diffusion gel method (Ouchterlony), was used as an index for estimating degree of serological correspondence in selected taxa of the Magnoliaceae. Proteins were saline extracted from seeds of 13 species and one variety of Magnolia, from four populations of M. virginiana, and from one species each of Manglietia, Michelia, Talauma, and Liriodendron. Serologically, Magnolia, Manglietia, Michelia, and Talauma can be grouped together, with the greatest serological similarity being between Magnolia and Michelia. Liriodendron was quite distinct from the other genera, although all have one antigen-antibody component in common. Although interspecific tests in Magnolia placed M. obovata and M. tripetala very close together, they were distinguishable by some of the precipitin tests. There was very little serological correspondence between M. portoricensis and the other 12 species of Magnolia tested; in fact, the correspondence was less ...

Wound regeneration in the lamina of magnoliaceous leaves

Abstract: Reparative tissue was observed around healed natural wounds in leaves of 53 species of 9 of the 12 magnoliaceous genera: Aromadendron, Elmerrillia, Kmeria, Liriodendron, Magnolia, Manglietia, Michelia, Paramichelia, and Talauma. Additional evidence was obtained from wounding experiments carried out on attached leaves of two evergreen species, Magnolia grandiflora and Michelia figo. Regeneration was studied over a 6-week period. Evergreen leaves produced more abundant regenerative tissues than deciduous leaves; a narrow-to-broad periderm forms consistently in all evergreen magnoliaceous leaves around wounds, but wounds in the deciduous species invoke little or no periderm development. The healing process in these leaves consists of three steps: (1) cells die along the wound, and cells immediately adjacent become physiologically altered; (2) a callus forms by randomly oriented cell divisions throughout mesophyll, vein sheath, and (in most species) epidermis; and (3) a periderm eventually forms in the band o...