Topic
Molecular breeding
About: Molecular breeding is a research topic. Over the lifetime, 2120 publications have been published within this topic receiving 56908 citations.
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TL;DR: In this paper, a review illustrates the potential of high-throughput sequencing in drug discovery, drug development, and large-scale production of plant natural products, and envisions its future development and applications in medicinal plant transcriptome studies.
Abstract: Preclinical Research
Two alternative approaches to medicinal plant transcriptome analysis, RNA sequencing (RNA-seq) and digital gene expression (DGE), are sequence based and have become increasingly popular due to rapid developments in high-throughput sequencing technologies. Among the high-throughput sequencing techniques, the 454 pyrosequencing and Illumina sequencing platforms are the first to be commercially available and established and thus widely used in various fields of medicinal plant transcriptome research. Metabolic pathway analysis of medicinal plants and molecular marker mining for molecular breeding can be facilitated and accelerated by RNA-seq. DGE provides novel insights into biochemical mechanisms in medicinal plants. This review illustrates the potential of high-throughput sequencing in drug discovery, drug development, and large-scale production of plant natural products, and envisions its future development and applications in medicinal plant transcriptome studies.
23 citations
TL;DR: The fundamental concepts, critical considerations and limitations of AM focusing on rice, and the importance of accurate phenotypic data are reviewed, covering practical consideration for breeders and connecting GWAS results in actual rice molecular breeding programs are covered.
Abstract: In the last decade, association mapping (AM) has become a well-established method to detect genes and quantitative trait loci (QTLs) associated with agronomically important traits. The identificati...
23 citations
TL;DR: An overview of genomic research in blueberry is provided, with a focus on expressed sequence tag/transcriptome sequencing efforts.
Abstract: Blueberry is a high-value crop with recognized nutritional characteristics that has led to an increase in consumer demand over the last several years. With its increasing agricultural and commercial importance, genetic and genomic tools have recently become available for use in characterizing its genetic diversity and in molecular breeding strategies. Here, we provide an overview of genomic research in blueberry, with a focus on expressed sequence tag/transcriptome sequencing efforts. These resources are already providing novel insights into various biological processes from large-scale expression studies, such as microarrays, elucidation of phylogenetic relationships, and development of molecular markers and genetic linkage maps. Future blueberry breeding programs should benefit greatly from these new genomic tools.
23 citations
TL;DR: The polymorphic alleles of each polymorphic locus were sequenced, and the results showed that most polymorphisms were due to variations of SSR repeat motifs.
Abstract: Simple sequence repeats (SSRs) are among the most important markers for population analysis and have been widely used in plant genetic mapping and molecular breeding. Expressed sequence tag-SSR (EST-SSR) markers, located in the coding regions, are potentially more efficient for QTL mapping, gene targeting, and marker-assisted breeding. In this study, we investigated 51,694 nonredundant unigenes, assembled from clean reads from deep transcriptome sequencing with a Solexa/Illumina platform, for identification and development of EST-SSRs in Chinese cabbage. In total, 10,420 EST-SSRs with over 12 bp were identified and characterized, among which 2744 EST-SSRs are new and 2317 are known ones showing polymorphism with previously reported SSRs. A total of 7877 PCR primer pairs for 1561 EST-SSR loci were designed, and primer pairs for twenty-four EST-SSRs were selected for primer evaluation. In nineteen EST-SSR loci (79.2%), amplicons were successfully generated with high quality. Seventeen (89.5%) showed polymorphism in twenty-four cultivars of Chinese cabbage. The polymorphic alleles of each polymorphic locus were sequenced, and the results showed that most polymorphisms were due to variations of SSR repeat motifs. The EST-SSRs identified and characterized in this study have important implications for developing new tools for genetics and molecular breeding in Chinese cabbage.
23 citations
06 Aug 2019
TL;DR: This paper discusses and summarizes various research findings on genetic improvement of Pleurotus and gives an outlook for future breeding programs.
Abstract: Pleurotus is an important genus comprising several edible species of great commercial significance. These species are grown all across the world. The production areas of Pleurotus mainly belong to the Asian part and are gaining wide popularity across the globe owing to their promising nutritional gains. The demand for improved strains with high productivity has also been rising. The genetic improvement in Pleurotus started with a simple selection technique, which later utilized hybridization (intraspecific, interspecific and intergeneric) and mutation breeding. The traits such as productivity, sporelessness and quality improvement are important objectives on which most of the works have been done so far. However, new generation approaches such as molecular breeding, genetic transformation and genome editing techniques also added pace to the present improvement process. Hitherto, seven species of Pleurotus have been sequenced and a sizable data has been generated that can be used in further breeding programs. This paper discusses and summarizes various research findings on genetic improvement of Pleurotus and gives an outlook for future breeding programs.
23 citations